Document Detail

Bactericidal function of alveolar macrophages in mechanically ventilated rabbits.
MedLine Citation:
PMID:  16474095     Owner:  NLM     Status:  MEDLINE    
Protective ventilation strategies have been universally embraced because of reduced mortality. We tested the hypothesis that tidal volume (VT) in an in vivo model of mechanical ventilation would modulate bactericidal function of alveolar macrophages (AMs). Adult New Zealand White rabbits were mechanically ventilated for 4 h with a VT of 6 ml/kg (low) or a VT of 12 ml/kg (traditional), with each group receiving 3 cm H2O positive end-expiratory pressure with and without intratracheal lipopolysaccharide (LPS) instillation (20 mg/kg). AMs were isolated from bronchoalveolar lavage fluid taken from the whole left lung and used for bacterial killing assays. There were no significant differences in steady-state levels of nitrite or AM phagocytosis and killing of Klebsiella pneumoniae, although these values trended to be slightly higher in the traditional VT group. However, bronchoalveolar lavage fluid protein concentrations were significantly increased in traditional VT groups receiving LPS compared with animals ventilated with a low VT (1,407.8 +/- 121.4 versus 934.7 +/- 118.2; P < 0.001). Lung wet:dry weight ratio in the traditional VT group was increased when compared with the low VT group without LPS (7.3 +/- 0.4 versus 6.1 +/- 0.3, respectively; P < 0.05). Additionally, IL-8 expression was significantly greater under conditions of LPS treatment and mechanical ventilation at VT of 12 ml/kg. These results suggest that the traditional ventilator approach (12 ml/kg VT) in a model of in vivo mechanical ventilation results in lung pathology without affecting AM antibacterial function.
Nina G Hall; Yuliang Liu; Judy M Hickman-Davis; Glenda C Davis; Carpantato Myles; Eric J Andrews; Sadis Matalon; John D Lang
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2006-02-10
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  34     ISSN:  1044-1549     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-05-18     Completed Date:  2006-06-29     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  719-26     Citation Subset:  IM    
Department of Anesthesiology,The University of Alabama at Birmingham, Birmingham, AL 35233-6810, USA.
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MeSH Terms
Bronchoalveolar Lavage Fluid / chemistry,  cytology
Extravascular Lung Water / metabolism
Interleukin-8 / analysis,  metabolism
Klebsiella pneumoniae / isolation & purification
Lung / immunology*,  metabolism
Lung Diseases / etiology,  immunology*,  metabolism
Macrophages, Alveolar / immunology*,  microbiology
Nitrites / analysis
Positive-Pressure Respiration
Proteins / analysis
Respiration, Artificial / methods*
Tidal Volume
Tumor Necrosis Factor-alpha / analysis,  metabolism
Grant Support
Reg. No./Substance:
0/Interleukin-8; 0/Lipopolysaccharides; 0/Nitrites; 0/Proteins; 0/Tumor Necrosis Factor-alpha

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