Document Detail


Bactericidal activity of a synthetic peptide (CG 117-136) of human lysosomal cathepsin G is dependent on arginine content.
MedLine Citation:
PMID:  8890249     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The importance of individual amino acids in mediating the broad-spectrum bactericidal action of a 20-mer amphipathic, cationic peptide (CG 117-136) of human lysosomal cathepsin G was determined by using a single amino acid replacement strategy. This strategy revealed an important role for arginine because loss of any of the four arginine residues in CG 117-136 due to substitution with alanine, citrulline, or lysine residues resulted in a reduction of its bactericidal activity against both Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 33593. However, the replacement of a single alanine residue in CG 117-136 with arginine, but not glutamic acid, enhanced the activity of CG 117-136 against both P. aeruginosa and S. aureus. The importance of certain bulky, nonpolar amino acids for the bactericidal activity of CG 117-136 was also evident, since their substitutions by alanine diminished bactericidal activity. Accordingly, contributions of hydrophobic amino acids and structural considerations of the guanidinium side chain of arginine are major determinants in the broad-spectrum antimicrobial action of CG 117-136.
Authors:
W M Shafer; F Hubalek; M Huang; J Pohl
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  64     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-01-06     Completed Date:  1997-01-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4842-5     Citation Subset:  IM    
Affiliation:
Laboratories of Microbial Pathogenesis, Research Service, Department of Veterans Affairs Medical Center, Decatur, Georgia 30033, USA. wshafer@unix.cc.emory.edu.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Anti-Bacterial Agents / chemistry,  pharmacology*
Arginine / analysis*
Cathepsin G
Cathepsins / chemistry*
Colony Count, Microbial
Humans
Lysosomes / enzymology*
Molecular Sequence Data
Peptides / chemistry,  pharmacology*
Proteins / chemistry,  pharmacology*
Pseudomonas aeruginosa / drug effects*
Serine Endopeptidases
Staphylococcus aureus / drug effects*
Grant Support
ID/Acronym/Agency:
AI-21150/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/CG 117136; 0/Peptides; 0/Proteins; 74-79-3/Arginine; EC 3.4.-/Cathepsins; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.20/CTSG protein, human; EC 3.4.21.20/Cathepsin G
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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