Document Detail

Bactericidal activity of phosphoric acid, citric acid, and EDTA solutions against Enterococcus faecalis.
MedLine Citation:
PMID:  18554953     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: The objectives of this study were to evaluate the minimal bactericidal concentration (MBC) for Enterococcus faecalis of phosphoric acid, citric acid, and ethylenediaminetetraacetic acid (EDTA) solutions, and to determine the contact time required for 2.5% and 5% phosphoric acid, 10% and 25% citric acid, and 17% EDTA to exert bactericidal activity. STUDY DESIGN: Bactericidal activity was tested by means of the dilution neutralization method in accordance with BS-EN-1040:2005 norm, using contact times of 0.5 to 60 minutes. RESULTS: The MBCs of citric and phosphoric acid were 20% and 2.5%, respectively. EDTA solution lacks bactericidal activity, even after 60 minutes of contact. The 2.5% and 5% phosphoric acid solutions required 5- and 3-minute contact times, and the 10% and 25% citric acid solutions required 10- and 3-minute contact times, respectively. CONCLUSIONS: Phosphoric acid revealed bactericidal activity against E. faecalis and required less time than citric acid to exert its activity.
María Teresa Arias-Moliz; Carmen María Ferrer-Luque; Elena Espigares-Rodríguez; José Liébana-Ureña; Miguel Espigares-García
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Publication Detail:
Type:  Journal Article     Date:  2008-06-13
Journal Detail:
Title:  Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics     Volume:  106     ISSN:  1528-395X     ISO Abbreviation:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-22     Completed Date:  2008-10-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508562     Medline TA:  Oral Surg Oral Med Oral Pathol Oral Radiol Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e84-9     Citation Subset:  D; IM    
Department of Operative Dentistry, Faculty of Dentistry, University of Granada, Spain.
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MeSH Terms
Anti-Bacterial Agents / pharmacology*
Chelating Agents / administration & dosage,  pharmacology
Citric Acid / administration & dosage,  pharmacology*
Edetic Acid / administration & dosage,  pharmacology*
Enterococcus faecalis / drug effects*
Materials Testing
Microbial Sensitivity Tests
Phosphoric Acids / administration & dosage,  pharmacology*
Root Canal Irrigants / administration & dosage,  pharmacology*
Time Factors
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Chelating Agents; 0/Phosphoric Acids; 0/Root Canal Irrigants; 60-00-4/Edetic Acid; 7664-38-2/phosphoric acid; 77-92-9/Citric Acid

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