Document Detail

Bactericidal effect of photodynamic therapy using Na-pheophorbide a: evaluation of adequate light source.
MedLine Citation:
PMID:  19715436     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To evaluate the efficacy of photodynamic therapy (PDT) against methicillin resistant-Staphylococcus aureus (MRSA) by selecting different light sources for irradiation and combining them with the photosensitizer Na-Pheophorbide a (Na-Phde a). BACKGROUND: The treatment of drug-resistant bacterial infection is a serious issue. Recently, as a new clinical approach against septic arthritis, an experimental in vivo and in vitro model for the inactivation of MRSA by PDT using the photosensitizer Na-Phde a has been developed. MATERIALS AND METHODS: Na-Phde a solution (280 micromol/L) was mixed with MRSA strain bacterial inoculum. After 60 minutes, light was irradiated for 30 minutes using the following light sources: GaA1p semiconductor laser (300 mW, 670 nm), halogen lamp (75 W), xenon lamp (300 W) and fluorescent lamp (27 W). Bacterial growth was evaluated after 24 hours incubation in a blood agar culture. RESULTS: The semiconductor laser and halogen lamp groups showed perfect bactericidal effects after PDT. The xenon lamp and fluorescent lamp groups showed partial bactericidal effects. CONCLUSIONS: The results of this experiment showed that PDT using the combination of Na-Phde a with a semiconductor laser or halogen lamp showed a better bactericidal performance than with xenon or fluorescent lamps. These findings indicated that PDT using Na-Phde a could be a useful treatment for septic arthritis and soft tissue infection.
Toru Yamamoto; Takanori Iriuchishima; Shin Aizawa; Tatsumasa Okano; Bunsei Goto; Yu Nagai; Takashi Horaguchi; Junnosuke Ryu; Akiyoshi Saito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Photomedicine and laser surgery     Volume:  27     ISSN:  1557-8550     ISO Abbreviation:  Photomed Laser Surg     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-28     Completed Date:  2010-03-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101222340     Medline TA:  Photomed Laser Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  849-53     Citation Subset:  IM    
Nihon University School of Medicine, Department of Orthopaedic Surgery, Tokyo, Japan.
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MeSH Terms
Arthritis, Infectious / drug therapy
Chlorophyll / analogs & derivatives*,  pharmacology
Methicillin-Resistant Staphylococcus aureus / drug effects*
Photochemotherapy / instrumentation,  methods*
Radiation-Sensitizing Agents / pharmacology*
Soft Tissue Infections / drug therapy
Staphylococcal Infections / drug therapy*
Reg. No./Substance:
0/Radiation-Sensitizing Agents; 1406-65-1/Chlorophyll; 15664-29-6/pheophorbide a

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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