Document Detail


Bacterial uptake by the marine sponge Aplysina aerophoba.
MedLine Citation:
PMID:  17265004     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sponges (Porifera) are filter feeders that take up microorganisms from seawater and digest them by phagocytosis. At the same time, many sponges are known to harbor massive consortia of symbiotic microorganisms, which are phylogenetically distinct from those in seawater, within the mesohyl matrix. In the present study, feeding experiments were performed to investigate whether phylogenetically different bacterial isolates, hereafter termed "food bacteria," microbial seawater consortia, and sponge symbiont consortia are taken up and processed differently by the host sponge. Aplysina aerophoba retained high numbers of bacterial isolates and microbial seawater consortia with rates of up to 2.76 x 10(6) bacteria (g sponge wet weight)(-1) h(-1), whereas the retention of sponge symbionts was lower by nearly two orders of magnitude [5.37 x 10(4) bacteria (g sponge wet weight)(-1) h(-1)]. In order to visualize the processing of a food bacterium within sponge tissues, the green fluorescent protein-labeled Vibrio strain MMW1, which had originally been isolated from A. aerophoba, was constructed. Incubation of this strain with A. aerophoba and subsequent visualization in tissue cryosections showed its presence in the choanocytes and/or endopinacocytes lining the canals but, unlike latex beads, not in deeper regions of the mesohyl, which suggests digestion of the bacteria upon contact with the host. Denaturing gradient gel electrophoresis (DGGE) was performed on the incubation seawater to monitor the changes in phylogenetic composition after incubation of the sponge with either seawater or sponge symbiont consortia. However, the DGGE experiment provided no evidence for selective processing of individual lineages by the host sponge. In conclusion, this study extends early studies by Wilkinson et al. (Proc R Soc London B 220:519-528, 1984) that sponges, here A. aerophoba, are able to differentiate between food bacteria and their own bacterial symbionts.
Authors:
Markus Wehrl; Michael Steinert; Ute Hentschel
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microbial ecology     Volume:  53     ISSN:  0095-3628     ISO Abbreviation:  Microb. Ecol.     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-06-01     Completed Date:  2007-06-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7500663     Medline TA:  Microb Ecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  355-65     Citation Subset:  IM    
Affiliation:
Zentrum für Infektionsforschung, Röntgenring 11, Universität Würzburg, D-97070 Würzburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteria* / isolation & purification
Feeding Behavior*
Porifera / microbiology*,  physiology*
Seawater / microbiology
Symbiosis*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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