| Bacterial translocation to mesenteric lymph nodes is increased in cirrhotic rats with ascites. | |
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MedLine Citation:
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PMID: 7768390 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/AIMS: Cirrhotic patients are predisposed to develop spontaneous bacteremias and/or peritonitis, mainly caused by enteric bacteria. The aim of this study was to investigate if bacterial translocation, which is the passage of bacteria from the intestinal lumen to regional lymph nodes and/or the systemic circulation, is increased in a rat model of cirrhosis. METHODS: Rats were studied after 12-16 weeks of CCl4 inhalation, when samples of mesenteric lymph nodes, blood, liver, and spleen for standard bacteriologic cultures and a fragment of colon and liver for histology were obtained. Immunostaining of the cecum was performed using a polyclonal anti-Escherichia coli antibody. RESULTS: A significantly greater proportion of rats with cirrhosis and ascites (5 of 9; 56%) had positive mesenteric lymph node cultures compared with cirrhotics without ascites (0 of 9) and normal controls (0 of 12) (P < 0.01). In one cirrhotic rat, E. coli was isolated from both mesenteric lymph nodes and ascites. Rats with cirrhosis and ascites had significantly greater cecal submucosal edema and inflammation than rats with no ascites and controls. Immunoreactivity with E. coli was present in the cecal wall in 3 of 5 animals with E. coli translocation to mesenteric lymph nodes. CONCLUSIONS: In cirrhotic rats, bacterial translocation is increased after the development of ascites and may be a major factor in the development of spontaneous infections in cirrhosis. |
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Authors:
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G Garcia-Tsao; F Y Lee; G E Barden; R Cartun; A B West |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Gastroenterology Volume: 108 ISSN: 0016-5085 ISO Abbreviation: Gastroenterology Publication Date: 1995 Jun |
Date Detail:
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Created Date: 1995-06-30 Completed Date: 1995-06-30 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0374630 Medline TA: Gastroenterology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1835-41 Citation Subset: AIM; IM |
Affiliation:
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Hepatic Hemodynamic Laboratory, West Haven Veterans Affairs Medical Center, Connecticut, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Ascites / microbiology Bacterial Physiological Phenomena* Liver Cirrhosis, Experimental / microbiology* Lymph Nodes / microbiology* Male Mesentery Movement Peritonitis / etiology Rats Rats, Sprague-Dawley |
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