Document Detail

Bacterial transformation (kushner method).
MedLine Citation:
PMID:  21374199     Owner:  NLM     Status:  In-Data-Review    
The principle of this procedure is very similar to that of the standard calcium chloride method, (see Chapter 34 ) i.e., exponential phase cells are harvested and washed with a solution containing divalent cations. This renders the cells competent, which simply means they are now able to take up DNA from the solution. After mixing the competent cells with the DNA, they are subjected to a heat shock to promote the uptake of DNA, presumably by affecting the physical state of the lipids in the membrane.
J W Dale; P J Greenaway
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  2     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  1985  
Date Detail:
Created Date:  2011-03-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  241-4     Citation Subset:  -    
Department of Microbiology, University of Surrey, Guildford, Surrey.
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