| Bacterial probiotic modulation of dendritic cells. | |
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MedLine Citation:
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PMID: 15155633 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intestinal dendritic cells are continually exposed to ingested microorganisms and high concentrations of endogenous bacterial flora. These cells can be activated by infectious agents and other stimuli to induce T-cell responses and to produce chemokines which recruit other cells to the local environment. Bacterial probiotics are of increasing use against intestinal disorders such as inflammatory bowel disease. They act as nonpathogenic stimuli within the gut to regain immunologic quiescence. This study was designed to determine the ability of a bacterial probiotic cocktail VSL#3 to alter cell surface antigen expression and cytokine production in bone marrow-derived dendritic cell-enriched populations. Cell surface phenotype was monitored by monoclonal fluorescent antibody staining, and cytokine levels were quantitated by enzyme-linked immunosorbent assay. High-dose probiotic upregulated the expression of C80, CD86, CD40, and major histocompatibility complex class II I-Ad. Neither B7-DC or B7RP-1 was augmented after low-dose probiotic or Lactobacillus casei treatment, but B7RP-1 showed increased expression on dendritic cells stimulated with the gram-negative bacterium Escherichia coli. Functional studies showed that probiotic did not enhance the ability of dendritic cells to induce allogeneic T-cell proliferation, as was observed for E. coli. Substantial enhancement of interleukin-10 release was observed in dendritic cell-enriched culture supernatants after 3 days of probiotic stimulation. These results demonstrate that probiotics possess the ability to modulate dendritic cell surface phenotype and cytokine release in granulocyte-macrophage colony-stimulating factor-stimulated bone marrow-derived dendritic cells. Regulation of dendritic cell cytokines by probiotics may contribute to the benefit of these molecules in treatment of intestinal diseases. |
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Authors:
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Maureen Drakes; Thomas Blanchard; Steven Czinn |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Infection and immunity Volume: 72 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-05-24 Completed Date: 2004-06-23 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: United States |
Other Details:
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Languages: eng Pagination: 3299-309 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA. mld19@cwru.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD / metabolism* Antigens, CD40 / metabolism Antigens, CD80 / metabolism Antigens, CD86 Cells, Cultured Dendritic Cells / immunology* Escherichia coli / physiology Female Gene Expression Regulation* Gram-Positive Bacteria / physiology* Histocompatibility Antigens Class II / metabolism* Interleukin-10 / metabolism* Membrane Glycoproteins / metabolism Mice Mice, Inbred BALB C Mice, Inbred C57BL Probiotics / administration & dosage* |
| Grant Support | |
ID/Acronym/Agency:
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DK-046461/DK/NIDDK NIH HHS; DK-57756/DK/NIDDK NIH HHS; T32-A152067-02//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Antigens, CD40; 0/Antigens, CD80; 0/Antigens, CD86; 0/Cd86 protein, mouse; 0/Histocompatibility Antigens Class II; 0/Membrane Glycoproteins; 130068-27-8/Interleukin-10 |
| Comments/Corrections | |
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