Document Detail


Bacterial monocultures, propionate, butyrate and H(2) O(2) modulate the expression, secretion and structure of the fasting-induced adipose factor in gut epithelial cell lines.
MedLine Citation:
PMID:  21518214     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Previous research showed that an intestinal microbial community represses the fasting-induced adipose factor (FIAF) in the gut epithelium, thereby increasing fat storage in the host. This study was designed to investigate the overall effect of different bacterial species and metabolites on FIAF in intestinal (Caco-2, HT-29 and HCT-116) and hepatic (HepG2) cancer cell lines. First, we showed that FIAF was present in different isoforms, and secreted as N-glycosylated proteins, exclusively at the basal side of the cell monolayer. Second, co-incubation of cell lines with bacterial monocultures and metabolites altered both FIAF production and isoform appearance. Propionate and/or butyrate treatment increased FIAF expression and cleavage in all tested cell lines. In contrast, different bacteria induced cell line-specific FIAF modulation. Clostridium perfringens induced FIAF isoform changes in Caco-2 cells. Enterococcus faecalis and Bacteroides thetaiotaomicron treatment resulted in cell line-specific FIAF increases, whereas Escherichia coli significantly decreased FIAF expression in HCT-116 cells. Treatment with H(2) O(2) and peroxide-producing E. faecalis strains induced FIAF isoform changes in Caco-2 cells. Since bacteria and bacterial metabolites alter both FIAF production and isoform appearance, further investigation may reveal an important role for bacteria in FIAF-regulated physiological processes, such as cell differentiation and fat metabolism.
Authors:
Charlotte Grootaert; Tom Van de Wiele; Ilse Van Roosbroeck; Sam Possemiers; Anne-Sophie Vercoutter-Edouart; Willy Verstraete; Marc Bracke; Barbara Vanhoecke
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-25
Journal Detail:
Title:  Environmental microbiology     Volume:  -     ISSN:  1462-2920     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883692     Medline TA:  Environ Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Society for Applied Microbiology and Blackwell Publishing Ltd.
Affiliation:
1Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, B-9000 Ghent, Belgium. Unity of Structural and Functional Glycobiology, UMR CNRS/USTL n°8576, Univ Lille Nord de France, 59655 Villeneuve d'Ascq, France. Laboratory of Experimental Cancer Research, University Hospital Ghent, B-9000 Ghent, Belgium.
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