Document Detail


Bacterial microcompartment shells of diverse functional types possess pentameric vertex proteins.
MedLine Citation:
PMID:  23456886     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bacterial microcompartments (MCPs) are large proteinaceous structures comprised of a roughly icosahedral shell and a series of encapsulated enzymes. MCPs carrying out three different metabolic functions have been characterized in some detail, while gene expression and bioinformatics studies have implicated other types, including one believed to perform glycyl radical-based metabolism of 1,2-propanediol (Grp). Here we report the crystal structure of a protein (GrpN), which is presumed to be part of the shell of a Grp-type MCP in Rhodospirillum rubrum F11. GrpN is homologous to a family of proteins (EutN/PduN/CcmL/CsoS4) whose members have been implicated in forming the vertices of MCP shells. Consistent with that notion, the crystal structure of GrpN revealed a pentameric assembly. That observation revived an outstanding question about the oligomeric state of this protein family: pentameric forms (for CcmL and CsoS4A) and a hexameric form (for EutN) had both been observed in previous crystal structures. To clarify these confounding observations, we revisited the case of EutN. We developed a molecular biology-based method for accurately determining the number of subunits in homo-oligomeric proteins, and found unequivocally that EutN is a pentamer in solution. Based on these convergent findings, we propose the name bacterial microcompartment vertex for this special family of MCP shell proteins.
Authors:
Nicole M Wheatley; Soheil D Gidaniyan; Yuxi Liu; Duilio Cascio; Todd O Yeates
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-04-08
Journal Detail:
Title:  Protein science : a publication of the Protein Society     Volume:  22     ISSN:  1469-896X     ISO Abbreviation:  Protein Sci.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-04-26     Completed Date:  2013-11-06     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  9211750     Medline TA:  Protein Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  660-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 The Protein Society.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / chemistry*,  genetics,  isolation & purification
Cloning, Molecular
Crystallography, X-Ray
Models, Molecular
Protein Multimerization
Rhodospirillum rubrum / chemistry*,  genetics
Grant Support
ID/Acronym/Agency:
GM007185/GM/NIGMS NIH HHS; P41GM103403/GM/NIGMS NIH HHS; P41RR015301/RR/NCRR NIH HHS; R01 AI081146/AI/NIAID NIH HHS; R01AI08114/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins
Comments/Corrections

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