Document Detail


Bacterial diversity in the saliva of patients with different oral hygiene indexes.
MedLine Citation:
PMID:  23207858     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The objective of the present study was to evaluate the bacterial diversity in the saliva of patients with different oral hygiene indexes using of two 16S rRNA gene libraries. Each library was composed of samples from patients with different averages of the differentiated Silness-Löe biofilm index: the first library (A) with an index between 1.0 and 3.0 (considered a high index) and the second library (B) between 0 and 0.5 (considered a low index). Saliva DNA was extracted and the 16S rRNA gene was amplified and cloned. The obtained sequences were compared with those stored at NCBI and RDP GenBank. The saliva of patients with high index presented five known genera - Streptococcus, Granulicatella, Gemella, Veillonella and Peptostreptococcus - and 33.3% of nonculturable bacteria grouped into 23 operational taxonomic units (OTUs). The saliva of patients with low index differed significantly from the first library (p=0.000) and was composed of 42 OTUs distributed into 11 known genera - Streptococcus, Granulicatella, Gemella, Veillonella, Oribacterium, Haemophilus, Escherichia, Neisseria, Prevotella, Capnocytophaga, Actinomyces - including 24.87% of nonculturable bacteria. It was possible to conclude that there is greater bacterial diversity in the saliva of patients with low dental plaque in relation to patients with high dental plaque.
Authors:
Juliana Vianna Pereira; Luciana Leomil; Fabíola Rodrigues-Albuquerque; José Odair Pereira; Spartaco Astolfi-Filho
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Brazilian dental journal     Volume:  23     ISSN:  1806-4760     ISO Abbreviation:  Braz Dent J     Publication Date:  2012  
Date Detail:
Created Date:  2012-12-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214652     Medline TA:  Braz Dent J     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  409-16     Citation Subset:  D    
Affiliation:
Multidisciplinary Support Center, Federal University of Amazonas, Manaus, AM, Brazil.
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