Document Detail

Bacterial diversity of the Inner Mongolian Baer Soda Lake as revealed by 16S rRNA gene sequence analyses.
MedLine Citation:
PMID:  15064989     Owner:  NLM     Status:  MEDLINE    
Bacterial diversity associated with Baer Soda Lake in Inner Mongolia of China was investigated using a culture-independent method. Bacterial 16S rRNA gene libraries were generated using bacterial oligonucleotide primers, and 16S rRNA gene sequences of 58 clones were analyzed phylogenetically. The library was dominated by 16S rDNAs of Gram-negative bacteria (24% alpha-Proteobacteria, 31% beta-Proteobacteria, 33% gamma-Proteobacteria, and 2% delta-Proteobacteria), with a lower percentage of clones corresponding to Gram-positive bacteria. Forty cloned sequences were similar to that of known bacterial isolates (> 97% sequence similarity), represented by the species of the genera Brevundimonas, Comamonas, Alcaligenes, Stenotrophomonas, and Klebsiella. Eighteen cloned sequences showed less affiliation with known taxa (< 97% sequence similarity) and may represent novel taxa.
Yanhe Ma; Weizhou Zhang; Yanfen Xue; Peijin Zhou; Antonio Ventosa; William D Grant
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-10-01
Journal Detail:
Title:  Extremophiles : life under extreme conditions     Volume:  8     ISSN:  1431-0651     ISO Abbreviation:  Extremophiles     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-04-05     Completed Date:  2004-10-05     Revised Date:  2014-05-09    
Medline Journal Info:
Nlm Unique ID:  9706854     Medline TA:  Extremophiles     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  45-51     Citation Subset:  IM; S    
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MeSH Terms
Bacteria / classification,  genetics*,  isolation & purification*
Base Sequence
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Fresh Water / microbiology*
Genes, Bacterial*
Genetic Variation
Gram-Positive Bacteria / classification,  genetics,  isolation & purification
Hydrogen-Ion Concentration
Proteobacteria / classification,  genetics,  isolation & purification
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Reg. No./Substance:
0/Carbonates; 0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S; 45P3261C7T/sodium carbonate

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