Document Detail


Bacterial communities associated with retail alfalfa sprouts.
MedLine Citation:
PMID:  18236685     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fresh produce, including salad, is increasingly implicated in foodborne outbreaks. Although studies have been carried out to detect specific human pathogens from fresh produce, the total bacterial community associated with fresh produce is poorly understood. In this study, we characterized the bacterial community associated with alfalfa sprouts, using a culture-independent method. Four retail-purchased alfalfa sprout samples were obtained from different producers, and the bacterial community associated with each sample was determined by 16S rDNA profiling. Our results indicate that alfalfa sprouts sampled in our study shared significant similarities in their bacterial communities. Proteobacteria was the dominant phylum detected from all alfalfa sprout samples, with Enterobacteriaceae, Oxalobacteraceae, Moraxellaceae, and Sphingomonadaceae as the most frequently detected families. These results indicate that growth conditions of alfalfa sprouts should be taken into consideration to prevent the proliferation of pathogenic proteobacteria such as Escherichia coli O157 and Salmonella.
Authors:
Cindy Loui; Grigor Grigoryan; Haohao Huang; Lee W Riley; Sangwei Lu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of food protection     Volume:  71     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-02-01     Completed Date:  2008-03-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  200-4     Citation Subset:  IM    
Affiliation:
Program in Infectious Diseases and Immunity, School of Public Health, 140 Warren Hall, University of California, Berkeley, California 94720, USA.
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MeSH Terms
Descriptor/Qualifier:
Colony Count, Microbial
Consumer Product Safety*
DNA, Bacterial / analysis
Enterobacteriaceae / growth & development,  isolation & purification*
Escherichia coli O157 / growth & development,  isolation & purification
Food Contamination / analysis*
Food Microbiology
Humans
Medicago sativa / microbiology*
Proteobacteria / growth & development,  isolation & purification*
Risk Assessment
Salmonella / growth & development,  isolation & purification
Grant Support
ID/Acronym/Agency:
5R01AI059523-03/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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