Document Detail


Bacterial bioluminescence as a bioassay for mycotoxins.
MedLine Citation:
PMID:  7181501     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The use of bacterial bioluminescence as a toxicological assay for mycotoxins was tested with rubratoxin B, zearalenone, penicillic acid, citrinin, ochratoxin A, PR-toxin, aflatoxin B1, and patulin. The concentrations of mycotoxins causing 50% light reduction (EC50) in Photobacterium phosphoreum were determined immediately and at 5 h after reconstitution of the bacteria from a freeze-dried state. Generally, less toxins were required to obtain an EC50 at 5 h. The effects of the above mycotoxins on bioluminescence were determined after 5, 10, 15, and 20 min of incubation with the bacterial suspensions. The concentration of rubratoxin B necessary to elicit an EC50 increased with time, whereas the concentration of citrinin, penicillic acid, patulin, and PR-toxin necessary decreased with time. There was very little change in the concentration of zearalenone, aflatoxin B1, and ochratoxin A required to elicit an EC50 with time. The bacterial bioluminescence assay was most sensitive to patulin and least sensitive to rubratoxin B.
Authors:
I E Yates; J K Porter
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  44     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1982 Nov 
Date Detail:
Created Date:  1983-02-14     Completed Date:  1983-02-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1072-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biological Assay*
Luminescence
Mycotoxins / analysis*,  pharmacology
Photobacterium / drug effects,  metabolism
Chemical
Reg. No./Substance:
0/Mycotoxins
Comments/Corrections

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