| Bacterial analysis of breast milk: a tool to differentiate Raynaud's phenomenon from infectious mastitis during lactation. | |
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MedLine Citation:
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PMID: 19330378 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Lactational Raynaud's syndrome may be misdiagnosed as infectious mastitis on the basis of the breast pain. The objective of this work was to elucidate if microbiological analysis of milk may contribute to the differentiation of both conditions. Ten lactating women clinically diagnosed by Spanish lactation consultants were included in the study. Of these, five suffered from mastitis and the remaining five suffered from Raynaud's syndrome. Breast milk samples were inoculated on diverse culture media. Seventy isolates were selected and identified by 16S rDNA PCR sequencing. Parallel, PCR-DGGE and quantitative real-time PCR were used to assess the presence of bacterial DNA in the samples. Neither bacteria nor yeasts could be detected in the milk samples provided by the women suffering from Raynaud's syndrome. In contrast, large numbers of bacteria were isolated from those with infectious lactational mastitis. Globally, the levels of bacterial DNA were significantly higher in the milk of mastitis-suffering women. Bacteriological analysis of milk can be an useful tool to facilitate the differential diagnosis between the infectious mastitis and Raynaud's syndrome during lactation. |
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Authors:
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Susana Delgado; M Carmen Collado; Leonides Fernández; Juan M Rodríguez |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-28 |
Journal Detail:
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Title: Current microbiology Volume: 59 ISSN: 1432-0991 ISO Abbreviation: Curr. Microbiol. Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2009-06-08 Completed Date: 2009-07-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7808448 Medline TA: Curr Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 59-64 Citation Subset: IM |
Affiliation:
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Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, Madrid, Spain. sdelgado@vet.ucm.es |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/FM992866; FM992867; FM992868; FM992869; FM992870 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Bacteria / classification, genetics, isolation & purification* DNA, Bacterial / genetics DNA, Ribosomal / genetics Female Humans Lactation* Mastitis / microbiology* Milk, Human / microbiology* Molecular Sequence Data RNA, Ribosomal, 16S / genetics Raynaud Disease / microbiology* Young Adult |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/DNA, Ribosomal; 0/RNA, Ribosomal, 16S |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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