| Bacterial and Salivary Biomarkers Predict the Gingival Inflammatory Profile. | |
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MedLine Citation:
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PMID: 21563952 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: The aim of this human investigation was to explore the relationship of gingivitis with salivary biomarkers, periodontal pathogens and interleukin (IL)-1 polymorphism following a transient inflammatory burden. Methods: Thirty healthy human subjects were randomized by IL-1 genotype status to control for potential influences of this particular single nucleotide polymorphism on the inflammatory profile. Oral hygiene practices ceased for 21d to induce gingivitis (induction) after which home care was reinstated until 35d (resolution). Clinical parameters included plaque (PI) and gingival (GI) indices, and papillary bleeding scores (PBS). Levels and proportions of 40 subgingival bacteria were determined using checkerboard DNA-DNA hybridization. Saliva was analyzed using a multiplex protein array for 30 biomarkers associated with host defense, inflammation, tissue destruction and angiogenesis. Results: Mean PI, GI and PBS values were significantly increased during induction and decreased during resolution as measured at 35d (p<0.01), although no differences were observed between IL-1 groups. Subjects were stratified as either "high" or "low" responders based on inflammatory response (high=GI>1.5; low=GI≤1.5). Baseline levels of salivary IL-6 and IL-8 demonstrated the highest ability to discriminate between "high" and "low" responders (area under the curve (AUC)=0.81 and 0.72, respectively). Salivary biomarkers, MMPs and bacterial biofilm were combined to generate receiver operating characteristic (ROC) curves. High levels of IL-6 and MMP-1 at baseline demonstrated the strongest ability to predict high responders (AUC=0.89, OR=17.0; 95% confidence interval 1.7-171.7). Conclusion: In this proof-of-concept investigation, we identified specific biomarker and microbial signatures that are associated with gingival inflammation (Clinical Trials.gov NCT 00980525). |
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Authors:
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Angie Lee; Carrie B Ghaname; Thomas M Braun; James V Sugai; Ricardo P Teles; Walter J Loesche; Kenneth S Kornman; William V Giannobile; Janet S Kinney |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-5-12 |
Journal Detail:
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Title: Journal of periodontology Volume: - ISSN: 1943-3670 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-5-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8000345 Medline TA: J Periodontol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Periodontics and Oral Medicine, Michigan Center for Oral Health Research, University of Michigan, Ann Arbor, MI, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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