Document Detail


Bacterial rRNA-targeted reverse transcription-PCR used to identify pathogens responsible for fever with neutropenia.
MedLine Citation:
PMID:  20351213     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study was to evaluate the clinical utility of bacterial rRNA-targeted reverse transcription-quantitative PCR (BrRNA RT-qPCR) assays for identifying the bacterial pathogens that cause fever with neutropenia in pediatric cancer patients, by comparing the bacterial detection rate of this technique with that of blood culture. One milliliter of blood was collected from pediatric patients who developed fever with neutropenia following cancer chemotherapy. BrRNA RT-qPCR was performed using 16 primer sets, each designed for a specific type of bacteria. The entire BrRNA RT-qPCR procedure took less than 5 h. Blood culture was performed at the same time, following the standard institutional procedure. Blood from 13 patients was collected during 23 febrile neutropenic episodes. Of these samples, bacteria were identified in 16 by BrRNA RT-qPCR (69.6%) and in 4 by blood culture (17.4%, P<0.001). In all 4 blood culture-positive samples, BrRNA RT-qPCR detected the same type of bacteria as that identified by culture. In 9 samples, more than 4 types of bacteria were identified simultaneously by BrRNA RT-qPCR, most of which were anaerobic bacteria known to be part of the gut flora. We conclude that BrRNA RT-qPCR could be useful in the diagnosis of fever with neutropenia, given its high bacterial detection rate, short turnaround time, and the small blood sample required compared with the standard blood culture techniques. Our findings also indicate that anaerobic intestinal bacteria, which are difficult to detect by standard culture techniques, may be responsible for some cases of febrile neutropenia.
Authors:
Sachi Sakaguchi; Masahiro Saito; Hirokazu Tsuji; Takashi Asahara; Oto Takata; Junya Fujimura; Satoru Nagata; Koji Nomoto; Toshiaki Shimizu
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-29
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  48     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-06     Completed Date:  2010-08-06     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1624-8     Citation Subset:  IM    
Affiliation:
Department of Pediatrics and Adolescent Medicine, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Bacteria / genetics,  isolation & purification*
Bacterial Infections / diagnosis*,  microbiology
Bacteriological Techniques / methods*
Blood / microbiology
Child
Child, Preschool
DNA Primers / genetics
Female
Fever of Unknown Origin / complications,  microbiology*
Humans
Immunocompromised Host
Infant
Male
Neoplasms / complications,  drug therapy
Neutropenia / complications,  microbiology*
RNA, Bacterial / genetics
RNA, Ribosomal / genetics*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Time Factors
Chemical
Reg. No./Substance:
0/DNA Primers; 0/RNA, Bacterial; 0/RNA, Ribosomal
Comments/Corrections

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