Document Detail


Bacterial motility and clustering guided by microcontact printing.
MedLine Citation:
PMID:  19891453     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Type IV pili are bacterial nanomotors that mediate two opposing behaviors on surfaces, spreading and clustering. Here we show that the velocity of motile Neisseria gonorrhoeae depends quantitatively on the fluidity of the phospholipid membrane surface. Using microcontact printing, we confined the surface motility to nonfluid islands within a fluid lipid membrane. On an array of islands, the transition from spreading to clustering was analyzed in real time and at the single cell level, showing that it was triggered by the number of bacteria (7.5 +/- 0.3) for small islands and by the surface density (56 +/- 2%) when the size of the island exceeded 25 microm(2).
Authors:
Claudia Holz; Dirk Opitz; Jan Mehlich; Bart Jan Ravoo; Berenike Maier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nano letters     Volume:  9     ISSN:  1530-6992     ISO Abbreviation:  Nano Lett.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-12-09     Completed Date:  2010-02-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088070     Medline TA:  Nano Lett     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4553-7     Citation Subset:  IM    
Affiliation:
Biology Department, Westfalische Wilhelms Universit?t, Schlossplatz 5, 48149 M?nster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Adhesion
Cell Culture Techniques / methods*
Lipid Bilayers / chemistry*
Motion
Neisseria gonorrhoeae / physiology*
Phospholipids / chemistry*
Chemical
Reg. No./Substance:
0/Lipid Bilayers; 0/Phospholipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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