Document Detail


Bacterial DNA signatures in carotid atherosclerosis represent both commensals and pathogens of skin origin.
MedLine Citation:
PMID:  23406581     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Infectious agents have been suggested to be involved in atherosclerosis. By using a novel subtraction broad-range PCR approach, we defined bacterial DNA signatures in surgically removed sterile carotid artery endarterectomy plaques of patients with carotid atherosclerosis. Eighty partial bacterial 16S rDNA nucleotide sequences from eight patients were studied. Furthermore, 34 clones representing 21 bacterial sequence-types from the reagents used for DNA extraction and PCR amplification were determined. After subtraction of these potential methodological contaminants, 23 bacterial sequence-types were considered as clinically relevant findings. The most prominent phylum, Actinobacteria, accounted for 74% of these relevant sequences. Furthermore, according to the Human Microbiome project database, interestingly, nearly all (94%) of the sequences were associated with the human skin microbiome.
Authors:
Jaana Renko; Katja A Koskela; Paul W Lepp; Niku Oksala; Mari Levula; Terho Lehtimäki; Tiina Solakivi; Tarja Kunnas; Simo Nikkari; Seppo T Nikkari
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of dermatology : EJD     Volume:  23     ISSN:  1952-4013     ISO Abbreviation:  Eur J Dermatol     Publication Date:    2013 Jan-Feb
Date Detail:
Created Date:  2013-03-12     Completed Date:  2013-10-18     Revised Date:  2014-01-08    
Medline Journal Info:
Nlm Unique ID:  9206420     Medline TA:  Eur J Dermatol     Country:  France    
Other Details:
Languages:  eng     Pagination:  53-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Actinobacteria / isolation & purification*
Aged
Aged, 80 and over
Carotid Artery Diseases / microbiology*
DNA, Bacterial / isolation & purification*
DNA, Ribosomal
Female
Humans
Male
Metagenome*
Middle Aged
Polymerase Chain Reaction
Sequence Analysis, DNA
Skin / microbiology*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Ribosomal

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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