| Bacterial DNA content in the intestinal wall from infants with necrotizing enterocolitis. | |
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MedLine Citation:
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PMID: 21683193 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: The objective of our study was to quantify mucosal bacterial DNA within specimens from neonates undergoing small bowel resection for necrotizing enterocolitis (NEC). METHODS: We obtained clinical information and pathologic specimens from all infants diagnosed with NEC who underwent surgical treatment at our institution from 1999 to 2008. Bacterial and human DNA were isolated from paraffin-embedded surgical specimens, and real time polymerase chain reaction was used to amplify bacterial and human genes. Linear regression was used to quantify the amount of human and bacterial DNA in our specimens. RESULTS: From a cohort of 50 infants, we identified 23 infants who underwent both surgical resection and subsequent intestinal reanastomosis. Thirteen (59%) of the neonates had Bell stage III NEC, and 9 (41%) had stage II. There was significantly more bacterial DNA in the resection specimens than in the reanastomosis specimens. This corresponds to a median (interquartile range) increase of 1.81 (1.11-4.69)-fold bacterial DNA in the resection specimen compared with the reanastomosis specimen (P < .05). CONCLUSION: There is more bacterial DNA in infants with acute NEC compared with the same infants after the NEC had clinically resolved. These findings underscore the potential relevance of adherent or invasive bacteria across the bowel wall in the pathogenesis of NEC. |
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Authors:
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Brian T Bucher; Lucas A McDuffie; Nurmohammad Shaikh; Phillip I Tarr; Barbara B Warner; Aaron Hamvas; Frances V White; Christopher R Erwin; Brad W Warner |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of pediatric surgery Volume: 46 ISSN: 1531-5037 ISO Abbreviation: J. Pediatr. Surg. Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-06-20 Completed Date: 2011-10-31 Revised Date: 2012-06-19 |
Medline Journal Info:
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Nlm Unique ID: 0052631 Medline TA: J Pediatr Surg Country: United States |
Other Details:
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Languages: eng Pagination: 1029-33 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Division of Pediatric Surgery, Department of Surgery, Washington University School of Medicine, St. Louis, MO 63110, USA. bucherb@wudosis.wustl.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Academic Medical Centers Age Factors Bacterial Translocation Biopsy, Needle Cohort Studies DNA, Bacterial / analysis* Enterocolitis, Necrotizing / microbiology*, pathology, surgery* Female Hospitals, Pediatric Humans Infant Infant, Newborn Intestinal Mucosa / microbiology* Male Retrospective Studies Reverse Transcriptase Polymerase Chain Reaction Risk Assessment Sensitivity and Specificity Tissue Embedding |
| Grant Support | |
ID/Acronym/Agency:
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1UH2AI083265-01/AI/NIAID NIH HHS; 5T32GM00879509/GM/NIGMS NIH HHS; UH3 AI083265-03/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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