Document Detail


Bacterial community diversity in undisturbed perhumid montane forest soils in Taiwan.
MedLine Citation:
PMID:  19727930     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The diversity and composition of soil bacterial communities in three topographic sites (summit, foot slope, and lakeshore) from subtropical montane forest ecosystem in Taiwan were examined by using 16S rRNA gene clone library analysis. This locality is temperate, perhumid, and has low soil acidity (pH < 4), which is an uncommon ecosystem in a monsoonal part of Southeast Asia. A total of 481 clones were sequenced and placed into ten phylogenetic groups according to their similarities to type strains of described organisms. Toposequence of the transect was investigated from summit to foot slope and at the lakeshore. More than 86% of the clones were affiliated with members of the Proteobacteria, Acidobacteria, and Actinobacteria. Within the Proteobacteria, the beta-Proteobacteria was the most abundant, then alpha-Proteobacteria and gamma-Proteobacteria. Based on the Shannon diversity index (H) analysis, the bacterial community in the foot slope was the most diverse (H = 0.86) and that in summit was the least diverse (H = 0.68). The composition and diversity of soil bacterial communities in the three sites suggested no trend with topographic change. Less than 20% of the sequences were Acidobacteria-affiliated clones. The low proportion of Acidobacteria observed may be related to the high soil moisture and anaerobic microhabitats. Moreover, Shannon diversity indices revealed these bacterial communities to have lower diversity than that of other temperate (H = 0.90) and tropical forest (H = 0.82) ecosystems. The extreme acidity of soil pH and high soil moisture of this forest may explain composition and reduced the diversity of these soil bacterial communities.
Authors:
Yu-Te Lin; Yu-Ju Huang; Sen-Lin Tang; William B Whitman; David C Coleman; Chih-Yu Chiu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-09-02
Journal Detail:
Title:  Microbial ecology     Volume:  59     ISSN:  1432-184X     ISO Abbreviation:  Microb. Ecol.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-03-11     Completed Date:  2010-04-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7500663     Medline TA:  Microb Ecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  369-78     Citation Subset:  IM    
Affiliation:
Biodiversity Research Center, Academia Sinica, Nankang, Taipei, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Bacteria / classification,  genetics*
Biodiversity*
DNA, Bacterial / genetics
Gene Library
Hydrogen-Ion Concentration
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Soil / analysis
Soil Microbiology*
Taiwan
Trees / microbiology*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/RNA, Ribosomal, 16S; 0/Soil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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