Document Detail

Bacteria and inflammatory cells in fetal membranes do not always cause preterm labor.
MedLine Citation:
PMID:  15659699     Owner:  NLM     Status:  MEDLINE    
Intrauterine infection has been frequently linked with preterm labor before 30 wk of human pregnancy. Many different species of organisms have been detected, leading to the suggestion that infection-induced preterm labor is a generic inflammatory response to organisms rather than a specific response to a limited number of pathogens. The detection of organisms by microbiological culture is a laborious and unreliable process, so the aim of this study was to harness modern molecular techniques to detect organisms in tissues from human pregnancy. A DNA probe specific for conserved regions of bacterial 16S ribosomal RNA sequence was designed and labeled with fluorescein for fluorescence in situ hybridization. Organisms were detected in the great majority (>80%) of fetal membranes after prolonged premature rupture of the fetal membranes and after preterm labor, which was consistent with previous data. Organisms were also detected in fetal membranes after preterm delivery without labor and in term deliveries (with or without labour). Inflammatory cells were found frequently in the amnion or chorion of preterm fetal membranes but not in term tissues. Our primary finding is that fluorescence in situ hybridization is an appropriate method to detect organisms in human fetal membranes. In addition, our data show that bacteria may be present in fetal membranes without necessarily causing an inflammatory response, so the mere presence of bacteria may not be sufficient to cause preterm labor.
Jennifer H Steel; Sotiris Malatos; Nigel Kennea; A David Edwards; Lynda Miles; Philip Duggan; Peter R Reynolds; Robert G Feldman; Mark H F Sullivan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-01-19
Journal Detail:
Title:  Pediatric research     Volume:  57     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-02-24     Completed Date:  2005-09-16     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  404-11     Citation Subset:  IM    
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MeSH Terms
Antigens, CD / metabolism
Bacteria / genetics,  metabolism*
Extraembryonic Membranes / cytology*,  microbiology*
Fetal Membranes, Premature Rupture
Gestational Age
Immune System / cytology*
In Situ Hybridization
In Situ Hybridization, Fluorescence / utilization
Nucleic Acid Probes / metabolism
Obstetric Labor, Premature*
Premature Birth
RNA, Bacterial / metabolism
RNA, Ribosomal, 16S / metabolism
Grant Support
MC_U120081323//Medical Research Council
Reg. No./Substance:
0/Antigens, CD; 0/Nucleic Acid Probes; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S

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