| Background light produces a recoverin-dependent modulation of activated-rhodopsin lifetime in mouse rods. | |
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MedLine Citation:
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PMID: 20107049 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Ca(2+)-binding protein recoverin is thought to regulate rhodopsin kinase and to modulate the lifetime of the photoexcited state of rhodopsin (Rh*), the visual pigment of vertebrate rods. Recoverin has been postulated to inhibit the kinase in darkness, when Ca(2+) is high, and to be released from the disk membrane in light when Ca(2+) is low, accelerating rhodopsin phosphorylation and shortening the lifetime of Rh*. This proposal has remained controversial, in part because the normally rapid turnoff of Rh* has made Rh* modulation difficult to study in an intact rod. To circumvent this problem, we have made mice that underexpress rhodopsin kinase so that Rh* turnoff is rate limiting for the decay of the rod light response. We show that background light speeds the decay of Rh* turnoff, and that this no longer occurs in mice that have had recoverin knocked out. This is the first demonstration in an intact rod that light accelerates Rh* inactivation and that the Ca(2+)-binding protein recoverin may be required for the light-dependent modulation of Rh* lifetime. |
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Authors:
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Ching-Kang Chen; Michael L Woodruff; Frank S Chen; Desheng Chen; Gordon L Fain |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: The Journal of neuroscience : the official journal of the Society for Neuroscience Volume: 30 ISSN: 1529-2401 ISO Abbreviation: J. Neurosci. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-01-28 Completed Date: 2010-07-08 Revised Date: 2011-05-17 |
Medline Journal Info:
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Nlm Unique ID: 8102140 Medline TA: J Neurosci Country: United States |
Other Details:
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Languages: eng Pagination: 1213-20 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, Virginia Commonwealth University, Richmond, Virginia 23298-0614, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism Calcium Signaling / physiology, radiation effects G-Protein-Coupled Receptor Kinase 1 / metabolism, radiation effects Light* Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout Mice, Transgenic Photic Stimulation Reaction Time / physiology, radiation effects Recoverin / metabolism, radiation effects* Retinal Rod Photoreceptor Cells / metabolism*, radiation effects* Rhodopsin / metabolism, radiation effects* Time Factors Vision, Ocular / physiology, radiation effects* |
| Grant Support | |
ID/Acronym/Agency:
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EY013811/EY/NEI NIH HHS; EY01844/EY/NEI NIH HHS; R01 EY001844-33/EY/NEI NIH HHS; R01 EY001844-34/EY/NEI NIH HHS; R01 EY013811-07/EY/NEI NIH HHS; R01 EY013811-09/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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135844-11-0/Recoverin; 7440-70-2/Calcium; 9009-81-8/Rhodopsin; EC 2.7.11.14/G-Protein-Coupled Receptor Kinase 1 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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