Document Detail


Bacillus thuringiensis protein transfer between rootstock and scion of grafted poplar.
MedLine Citation:
PMID:  22372666     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Bacillus thuringiensis (Bt) Cry1Ac protein is a toxin against different leaf-eating lepidopteran insects that attack poplar trees. In the present study, the mode of migration of the Bt-Cry1Ac protein within poplar grafts was investigated. Grafting was done using Pb29 (transgenic poplar 741 with cry1Ac genes), CC71 (transgenic poplar 741 with cry3A genes), non-transgenic poplar 741 and non-transgenic Populus tomentosa, either as scion or as rootstock. In order to detect migration of Bt-Cry1Ac protein from one portion of the graft union to different tissues in the grafted plant, ELISA analysis was employed to assess the content of Bt-Cry1Ac protein in the phloem, xylem, pith and leaves of the grafted poplar. To further verify migration of Bt-Cry1Ac protein, Clostera anachoreta larvae, which are susceptible to Bt-Cry1Ac protein, were fed leaves from the control graft (i.e., graft portion that originally did not contain Bt-Cry1Ac protein). The results showed that Bt-Cry1Ac protein was transported between rootstock and scion mainly through the phloem. Migration of Bt-Cry1Ac protein in the grafted union was also evidenced in that the leaves of the control graft did have a lethal effect on C. anachoreta larvae in laboratory feeding experiments.
Authors:
L Wang; M Yang; A Akinnagbe; H Liang; J Wang; D Ewald
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-28
Journal Detail:
Title:  Plant biology (Stuttgart, Germany)     Volume:  -     ISSN:  1438-8677     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101148926     Medline TA:  Plant Biol (Stuttg)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 German Botanical Society and The Royal Botanical Society of the Netherlands.
Affiliation:
 Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, Baoding, China  College of Horticulture, Hebei North University, Zhangjiakou, China  Department of Forestry & Wood Technology, Federal University of Technology, Akure, Nigeria  Institute of Forest Genetics, Johann Heinrich von Thuenen Institute Federal Research Institute for Rural Areas, Forestry and Fisheries, Waldsieversdorf, Germany.
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