Document Detail

A Bacillus subtilis spore coat polypeptide gene, cotS.
MedLine Citation:
PMID:  7545510     Owner:  NLM     Status:  MEDLINE    
A gene, cotS, encoding a spore coat polypeptide of Bacillus subtilis, was isolated from an EcoRI fragment (5.4 kb) of the chromosome by using synthetic oligonucleotide probes corresponding to the NH2-terminal amino acid sequence of Cot40-2 previously purified from the spore coat of B. subtilis. The nucleotide sequence (2603 bp) was determined and sequence analysis suggested the presence of two contiguous ORFs, ORF X and cotS, followed by the 5'-region of an additional ORF, ORF Y, downstream of cotS. The cotS gene is 1053 nucleotides long and encodes a polypeptide of 351 amino acids with a predicted molecular mass of 41083 Da. The predicted amino acid sequence was in complete agreement with the NH2-terminal amino acid sequence of Cot40-2. The orfX gene is 1131 nucleotides long and encodes a polypeptide of 377 amino acids with a predicted molecular mass of 42911 Da. The gene product of cotS was confirmed to be identical to Cot40-2 by SDS-PAGE and immunoblotting from Escherichia coli transformed with a plasmid containing the cotS region. Northern hybridization analysis indicated that a transcript of cotS and orfX appeared at about 5 h after the onset of sporulation. The transcriptional start point determined by primer extension analysis suggested that -10 and -35 regions are present upstream of orfX and are very similar to the consensus sequence for the sigma k-dependent promoter. Terminator-like sequences were not found in the DNA fragment (2603 bp) sequenced in this paper, which suggested that the cotS locus may be part of a multicistronic operon. The cotS gene is located between dnaB and degQ at about 270-275 degrees on the genetic map. Insertional mutagenesis of the cotS gene by introducing an integrative plasmid resulted in no alteration of growth or sporulation, and had no effect on germination or resistance to chloroform.
A Abe; H Koide; T Kohno; K Watabe
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Microbiology (Reading, England)     Volume:  141 ( Pt 6)     ISSN:  1350-0872     ISO Abbreviation:  Microbiology (Reading, Engl.)     Publication Date:  1995 Jun 
Date Detail:
Created Date:  1995-10-13     Completed Date:  1995-10-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9430468     Medline TA:  Microbiology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1433-42     Citation Subset:  IM    
Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
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MeSH Terms
Amino Acid Sequence
Bacillus subtilis / drug effects,  genetics*,  physiology
Bacterial Proteins / genetics*,  isolation & purification
Base Sequence
Chloroform / pharmacology
Chromosome Mapping
Chromosomes, Bacterial
Cloning, Molecular
Genes, Bacterial*
Molecular Sequence Data
Mutagenesis, Insertional
RNA, Bacterial / biosynthesis,  genetics
RNA, Messenger / biosynthesis,  genetics
Sequence Alignment
Sequence Homology
Spores, Bacterial
Reg. No./Substance:
0/Bacterial Proteins; 0/CotS protein, Bacillus subtilis; 0/RNA, Bacterial; 0/RNA, Messenger; 67-66-3/Chloroform

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