Document Detail

Bacillus subtilis ribonucleic acid polymerase mutants conditionally temperature sensitive at various stages of sporulation.
MedLine Citation:
PMID:  401499     Owner:  NLM     Status:  MEDLINE    
Rifampin-resistant mutants of Bacillus subtilis that are conditionally temperature sensitive during sporulation have been isolated and characterized. The mutants can grow at the same rate as the wild type at the nonpermissive temperature but cannot sporulate. Depending on the mutation, they are blocked at either stage 0 to I, II, II to III, or IV of sporulation. The mutants showed an altered pattern of RNA synthesis after the stage at which they were blocked. The effect of rifampin on the activity of enzymes from mutant vegetative cells and sporulating cells was significantly different, suggesting that the RNA polymerase from sporulating cells was different from the RNA polymerase of vegetative cells. These results suggest that the conformation of the RNA polymerase core plays an important role in determining correct transcription during sporulation.
C Sumida-Yasumoto; R H Doi
Related Documents :
10200269 - Oligoribonuclease is an essential component of the mrna decay pathway.
7731989 - In situ isolation of mrna from individual plant cells: creation of cell-specific cdna l...
7511419 - Poly(a)+ ribonucleic acids are enriched in spermatocyte nuclei but not in chromatoid bo...
10343139 - Interaction of putrescine with nuclear oligopeptides in the enterocyte-like caco-2 cells.
3021099 - Primary isolation and identification of avian rotaviruses from turkeys exhibiting signs...
21339059 - Spheroid confrontation assay: a simple method to monitor the three-dimensional migratio...
19347029 - Inactivation of ampk alters gene expression and promotes growth of prostate cancer cells.
4152249 - Selection for neuroblastoma cells that synthesize certain transmitters.
15466389 - Activated hepatic stellate cells express keratinocyte growth factor in chronic liver di...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  129     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1977 Jan 
Date Detail:
Created Date:  1977-02-26     Completed Date:  1977-02-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  433-44     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacillus subtilis / enzymology*,  metabolism,  physiology
Bacterial Proteins / biosynthesis
DNA-Directed RNA Polymerases / metabolism*
Drug Resistance, Microbial
Nucleic Acid Hybridization
RNA, Bacterial / biosynthesis
Rifampin / pharmacology
Spores, Bacterial
Reg. No./Substance:
0/Bacterial Proteins; 0/RNA, Bacterial; 13292-46-1/Rifampin; EC RNA Polymerases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Two polypeptides associated with the ribonucleic acid polymerase core of Bacillus subtilis during sp...
Next Document:  Two-dimensional restriction analysis of the Bacillus subtilis genome: gene purification and ribosoma...