| Bacillus subtilis ribonucleic acid polymerase mutants conditionally temperature sensitive at various stages of sporulation. | |
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MedLine Citation:
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PMID: 401499 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rifampin-resistant mutants of Bacillus subtilis that are conditionally temperature sensitive during sporulation have been isolated and characterized. The mutants can grow at the same rate as the wild type at the nonpermissive temperature but cannot sporulate. Depending on the mutation, they are blocked at either stage 0 to I, II, II to III, or IV of sporulation. The mutants showed an altered pattern of RNA synthesis after the stage at which they were blocked. The effect of rifampin on the activity of enzymes from mutant vegetative cells and sporulating cells was significantly different, suggesting that the RNA polymerase from sporulating cells was different from the RNA polymerase of vegetative cells. These results suggest that the conformation of the RNA polymerase core plays an important role in determining correct transcription during sporulation. |
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Authors:
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C Sumida-Yasumoto; R H Doi |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of bacteriology Volume: 129 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1977 Jan |
Date Detail:
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Created Date: 1977-02-26 Completed Date: 1977-02-26 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 433-44 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus subtilis
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enzymology*,
metabolism,
physiology Bacterial Proteins / biosynthesis DNA-Directed RNA Polymerases / metabolism* Drug Resistance, Microbial Genes Mutation Nucleic Acid Hybridization RNA, Bacterial / biosynthesis Rifampin / pharmacology Spores, Bacterial Temperature |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/RNA, Bacterial; 13292-46-1/Rifampin; EC 2.7.7.6/DNA-Directed RNA Polymerases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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