Document Detail


Bacillus subtilis ribonucleic acid polymerase mutants conditionally temperature sensitive at various stages of sporulation.
MedLine Citation:
PMID:  401499     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rifampin-resistant mutants of Bacillus subtilis that are conditionally temperature sensitive during sporulation have been isolated and characterized. The mutants can grow at the same rate as the wild type at the nonpermissive temperature but cannot sporulate. Depending on the mutation, they are blocked at either stage 0 to I, II, II to III, or IV of sporulation. The mutants showed an altered pattern of RNA synthesis after the stage at which they were blocked. The effect of rifampin on the activity of enzymes from mutant vegetative cells and sporulating cells was significantly different, suggesting that the RNA polymerase from sporulating cells was different from the RNA polymerase of vegetative cells. These results suggest that the conformation of the RNA polymerase core plays an important role in determining correct transcription during sporulation.
Authors:
C Sumida-Yasumoto; R H Doi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  129     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1977 Jan 
Date Detail:
Created Date:  1977-02-26     Completed Date:  1977-02-26     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  433-44     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacillus subtilis / enzymology*,  metabolism,  physiology
Bacterial Proteins / biosynthesis
DNA-Directed RNA Polymerases / metabolism*
Drug Resistance, Microbial
Genes
Mutation
Nucleic Acid Hybridization
RNA, Bacterial / biosynthesis
Rifampin / pharmacology
Spores, Bacterial
Temperature
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/RNA, Bacterial; 13292-46-1/Rifampin; EC 2.7.7.6/DNA-Directed RNA Polymerases
Comments/Corrections

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