Document Detail


Bacillus subtilis-mediated protection from Citrobacter rodentium-associated enteric disease requires espH and functional flagella.
MedLine Citation:
PMID:  22144475     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Commensals limit disease caused by invading pathogens; however, the mechanisms and genes utilized by beneficial microbes to inhibit pathogenesis are poorly understood. The attaching and effacing mouse pathogen Citrobacter rodentium associates intimately with the intestinal epithelium, and infections result in acute colitis. C. rodentium is used to model the human pathogens enterohemorrhagic Escherichia coli and enteropathogenic E. coli. To confirm that Bacillus subtilis, a spore-forming bacterium found in the gut of mammals, could reduce C. rodentium-associated disease, mice received wild-type B. subtilis spores and 24 h later were infected by oral gavage with pathogenic C. rodentium. Disease was assessed by determining the extent of colonic epithelial hyperplasia, goblet cell loss, diarrhea, and pathogen colonization. Mice that received wild-type B. subtilis prior to enteric infection were protected from disease even though C. rodentium colonization was not inhibited. In contrast, espH and hag mutants, defective in exopolysaccharides and flagellum production, respectively, did not protect mice from C. rodentium-associated disease. A motAB mutant also failed to protect mice from disease, suggesting that B. subtilis-mediated protection requires functional flagella. By expanding our current mechanistic knowledge of bacterial protection, we can better utilize beneficial microbes to prevent intestinal disease caused by pathogenic bacteria, ultimately reducing human disease. Our data demonstrate that wild-type B. subtilis reduced disease caused by C. rodentium infection through a mechanism that required espH and functional flagella.
Authors:
Sara E Jones; Katherine L Knight
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-12-05
Journal Detail:
Title:  Infection and immunity     Volume:  80     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-23     Completed Date:  2012-03-27     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  710-9     Citation Subset:  IM    
Affiliation:
Loyola University Chicago, Chicago, Illinois, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacillus subtilis / genetics*,  metabolism*
Bacterial Proteins / genetics,  metabolism*
Citrobacter rodentium / physiology*
Colon / microbiology,  pathology
Diarrhea
Enteritis / microbiology*
Gene Expression Regulation, Bacterial / physiology*
Goblet Cells
Intestinal Mucosa / pathology
Mice
Mutation
Spores, Bacterial
Time Factors
Grant Support
ID/Acronym/Agency:
NIAID 50260//PHS HHS; R01 AI050260/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins
Comments/Corrections

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