| The Bacillus subtilis cannibalism toxin SDP collapses the proton motive force and induces autolysis. | |
| | |
MedLine Citation:
|
PMID: 22469514 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Bacillus subtilis SDP is a peptide toxin that kills cells outside the biofilm to support continued growth. We show that purified SDP acts like endogenously produced SDP; it delays sporulation, and the SdpI immunity protein confers SDP resistance. SDP kills a variety of Gram-positive bacteria in the phylum Firmicutes, as well as Escherichia coli with a compromised outer membrane, suggesting it participates in defence of the B. subtilis biofilm against Gram-positive bacteria as well as cannibalism. Fluorescence microscopy reveals that the effect of SDP on cells differs from that of nisin, nigericin, valinomycin and vancomycin-KCl, but resembles that of CCCP, DNP and azide. Indeed, SDP rapidly collapses the PMF as measured by fluorometry and flow cytometry, which triggers the slower process of autolysis. This secondary consequence of SDP treatment is not required for cell death since the autolysin-defective lytC, lytD, lytE, lytF strain fails to be lysed but is nevertheless killed by SDP. Collapsing the PMF is an ideal mechanism for a toxin involved in cannibalism and biofilm defence, since this would incapacitate neighbouring cells by inhibiting motility and secretion of proteins and toxins. It would also induce autolysis in many Gram-positive species, thereby releasing nutrients that promote biofilm growth. |
| | |
Authors:
|
Anne Lamsa; Wei-Ting Liu; Pieter C Dorrestein; Kit Pogliano |
Related Documents
:
|
19166064 - Molecular characterization and typing of enrofloxacin-resistant clinical isolates of my... 12039884 - Response of escherichia coli hypermutators to selection pressure with antimicrobial age... 22632464 - Susceptibility of different mice strains to okadaic acid, a diarrhetic shellfish poison... 11404074 - Absence of mutation in the region (nt. 710-1010) of pbp4 gene in clinical isolates of s... 20860534 - Hiv-1 drug resistance transmission networks in southwest switzerland. 6686384 - The use of tick transmission by boophilus microplus to isolate pure strains of babesia ... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-04-02 |
Journal Detail:
|
Title: Molecular microbiology Volume: 84 ISSN: 1365-2958 ISO Abbreviation: Mol. Microbiol. Publication Date: 2012 May |
Date Detail:
|
Created Date: 2012-04-20 Completed Date: 2012-10-15 Revised Date: 2013-01-30 |
Medline Journal Info:
|
Nlm Unique ID: 8712028 Medline TA: Mol Microbiol Country: England |
Other Details:
|
Languages: eng Pagination: 486-500 Citation Subset: IM |
Copyright Information:
|
© 2012 Blackwell Publishing Ltd. |
Affiliation:
|
Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacillus subtilis
/
chemistry,
cytology*,
genetics,
metabolism* Bacteria / chemistry, drug effects Bacterial Proteins / genetics, metabolism Bacterial Toxins / metabolism*, toxicity Bacteriolysis* Proton-Motive Force* / drug effects |
| Grant Support | |
ID/Acronym/Agency:
|
AI095125/AI/NIAID NIH HHS; GM7240/GM/NIGMS NIH HHS; R01 AI095125/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Proteins; 0/Bacterial Toxins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mitochondrial superoxide mediates doxorubicin-induced keratinocyte apoptosis through oxidative modif...
Next Document: The effect of radixin knockdown on the expression and efflux function of MRP2 in SGC-7901 cells.