Document Detail

A Bacillus sphaericus Based Biosensor for Monitoring Nickel Ions in Industrial Effluents and Foods.
Jump to Full Text
MedLine Citation:
PMID:  17671626     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A microbial-based biosensor has been developed based on enzyme inhibition bioassay for monitoring the presence of Ni(II) in real-time samples. The sensing element is immobilized Bacillus sphaericus MTCC 5100 yielding urease enzyme. The transducer is an NH4+ ion selective electrode in conjunction with a potentiometer. Heavy metals are potentially toxic to human beings. Nickel is associated with causing adverse health effects such as dermatitis and vertigo, in humans. Toxicity is manifested by affecting T-cell system and suppressing the activity of natural killer cells. Nickel finds applications in electroplating, coinage, electrodes, jewellery, alloys. The foods rich in Ni(II) are nuts, beans, oats, and wheat. The range of Ni(II) detection by the developed biosensor is 0.03-0.68 nM (0.002-0.04 ppb) with a response time of 1.5 minutes. For application, the Ni(II) effluent was procured from an electroplating industrial unit and was found to have a concentration of 100.0 ppm Ni(II). In foods, wheat flour sample was acid digested and Ni(II) was specifically complexed in the presence of other cations, and had an Ni(II) concentration of 0.044 ppm. The developed system has a reliability of 91.5% and 90.6%, respectively, for the samples and could possibly replace the existing conventional techniques of analysis.
Authors:
Neelam Verma; Minni Singh
Related Documents :
24617026 - Estimation of glycaemic index of peach palm (bactris gasipaes) cooked fruits and chips,...
8757456 - Levels of cd, hg, as, cu, mn, and zn in japanese restaurant and box lunches.
12088376 - Food chain transfer of zinc within the ecosystems of old and modern metalliferous mine ...
14754576 - Assessing potential risk of heavy metal exposure from consumption of home-produced vege...
25094066 - A german version of the night eating questionnaire (neq): psychometric properties and c...
23941556 - Food losses and waste in china and their implication for water and land.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of automated methods & management in chemistry     Volume:  2006     ISSN:  1463-9246     ISO Abbreviation:  J Autom Methods Manag Chem     Publication Date:  2006  
Date Detail:
Created Date:  2007-08-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100885454     Medline TA:  J Autom Methods Manag Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  83427     Citation Subset:  -    
Affiliation:
Biosensor Development Laboratory Department of Biotechnology Punjabi University Patiala 147 002 India.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Autom Methods Manag Chem
ISSN: 1463-9246
ISSN: 1464-5068
Article Information
Download PDF
Copyright ? 2006 Neelam Verma and Minni Singh.
open-access: This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received Day: 13 Month: 12 Year: 2005
Accepted Day: 4 Month: 1 Year: 2006
Print publication date: Year: 2006
Electronic publication date: Day: 1 Month: 6 Year: 2006
Volume: 2006E-location ID: 83427
ID: 1903461
PubMed Id: 17671626
Publisher Item Identifier: S1463924606834279
DOI: 10.1155/JAMMC/2006/83427

A Bacillus sphaericus Based Biosensor for Monitoring Nickel Ions in Industrial Effluents and Foods
Neelam Verma1
Minni Singh1 Address: minni_singh@rediffmail.com
1Biosensor Development LaboratoryDepartment of BiotechnologyPunjabi UniversityPatiala147 002India


Article Categories:
  • Research Article


Previous Document:  Instrumentation and automated photometric titration procedure for total acidity determination in red...
Next Document:  Quantitative determination of the multicomponents with overlapping ultraviolet spectra using wavelet...