Document Detail


Bacillus nematocida sp. nov., a novel bacterial strain with nematotoxic activity isolated from soil in Yunnan, China.
MedLine Citation:
PMID:  15997705     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An endospore-forming bacterium, designated strain B-16T, was isolated from a forest soil sample in Yunnan, China. The isolate presented remarkable nematotoxic activity against nematode Panagrellus redivivus. The organism was strictly aerobic, motile, spore forming and rod shaped, catalase- and oxidase-positive. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major cellular fatty acid profiles were anteiso-C15:0 (48.67%), iso-C15:0 (13.45%), C16:0 (9.06%) and anteiso-Cl7:0 (8.29%). The DNA G+C content was 46%. Phylogenetic analyses based on 16S rDNA sequence revealed that isolate belongs to the genus Bacillus. Strain B-16T exhibited high 16S rDNA similarity with its closest neighbors Bacillus vallismortis (99.79%), B. subtilis (99.43%), B. atrophaeus (99.43%), B. amyloliquefaciens (99.36%), B. licheniformis (98.0%) and less than 97.0% with all the other relative type strains in the genus Bacillus. The phenotypic and genotypic characteristics and DNA-DNA relatedness data indicate that strain B-16T should be distinguished from all the relative species of genus Bacillus. Therefore, on the basis of the polyphasic taxonomic data presented, a new species of the genus Bacillus, B. nematocida, with the type strain B-16T ( = CGMCC 1128T) is proposed. The GenBank accession number for the sequence reported in this paper is AY820954.
Authors:
Xiao-Wei Huang; Qiu-Hong Niu; Wei Zhou; Ke-Qin Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Systematic and applied microbiology     Volume:  28     ISSN:  0723-2020     ISO Abbreviation:  Syst. Appl. Microbiol.     Publication Date:  2005 Jun 
Date Detail:
Created Date:  2005-07-06     Completed Date:  2005-09-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8306133     Medline TA:  Syst Appl Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  323-7     Citation Subset:  IM    
Affiliation:
Laboratory for Conservation and Utilization of Bio-Resources, Yunnan University, Kunming, Yunnan 650091, P.R. China.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AY820954
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MeSH Terms
Descriptor/Qualifier:
Aerobiosis
Animals
Bacillus / classification*,  cytology,  isolation & purification*,  physiology
Bacterial Typing Techniques
Base Composition
Catalase / analysis
China
DNA, Bacterial / chemistry,  isolation & purification
DNA, Ribosomal / chemistry,  isolation & purification
Fatty Acids / analysis,  isolation & purification
Genes, rRNA
Molecular Sequence Data
Movement
Nucleic Acid Hybridization
Oxidoreductases / analysis
Phylogeny
Quinones / analysis
RNA, Ribosomal, 16S / genetics
Rhabditida / microbiology*,  physiology
Sequence Analysis, DNA
Soil Microbiology*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Ribosomal; 0/Fatty Acids; 0/Quinones; 0/RNA, Ribosomal, 16S; EC 1.-/Oxidoreductases; EC 1.11.1.6/Catalase

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