Document Detail

Bacillus cereus induces permeability of an in vitro blood-retina barrier.
MedLine Citation:
PMID:  18268029     Owner:  NLM     Status:  MEDLINE    
Most Bacillus cereus toxin production is controlled by the quorum-sensing-dependent, pleiotropic global regulator plcR, which contributes to the organism's virulence in the eye. The purpose of this study was to analyze the effects of B. cereus infection and plcR-regulated toxins on the barrier function of retinal pigment epithelium (RPE) cells, the primary cells of the blood-retina barrier. Human ARPE-19 cells were apically inoculated with wild-type or quorum-sensing-deficient B. cereus, and cytotoxicity was analyzed. plcR-regulated toxins were not required for B. cereus-induced RPE cytotoxicity, but these toxins did increase the rate of cell death, primarily by necrosis. B. cereus infection of polarized RPE cell monolayers resulted in increased barrier permeability, independent of plcR-regulated toxins. Loss of both occludin and ZO-1 expression occurred by 8 h postinfection, but alterations in tight junctions appeared to precede cytotoxicity. Of the several proinflammatory cytokines analyzed, only interleukin-6 was produced in response to B. cereus infection. These results demonstrate the deleterious effects of B. cereus infection on RPE barrier function and suggest that plcR-regulated toxins may not contribute significantly to RPE barrier permeability during infection.
A L Moyer; R T Ramadan; J Thurman; A Burroughs; M C Callegan
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-02-11
Journal Detail:
Title:  Infection and immunity     Volume:  76     ISSN:  1098-5522     ISO Abbreviation:  Infect. Immun.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-19     Completed Date:  2008-04-17     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1358-67     Citation Subset:  IM    
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center,Oklahoma City, OK 73104, USA.
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MeSH Terms
Bacillus cereus / physiology*
Blood-Retinal Barrier / microbiology*,  pathology*
Cell Line
Protein Transport
Sodium-Potassium-Exchanging ATPase / metabolism
Tight Junctions / metabolism
Grant Support
P30 EY012190/EY/NEI NIH HHS; P30 EY012190-069006/EY/NEI NIH HHS; P30 EY012190-079006/EY/NEI NIH HHS; P30 EY012190-089006/EY/NEI NIH HHS; P30 EY012190-099006/EY/NEI NIH HHS; P30 EY012190-109006/EY/NEI NIH HHS; P30 EY12190/EY/NEI NIH HHS; R01 EY012985/EY/NEI NIH HHS; R01 EY012985-05A1/EY/NEI NIH HHS; R01 EY012985-06/EY/NEI NIH HHS; R01 EY012985-07/EY/NEI NIH HHS; R01 EY012985-08/EY/NEI NIH HHS; R01EY12985/EY/NEI NIH HHS
Reg. No./Substance:

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