Document Detail


The BRC repeats are conserved in mammalian BRCA2 proteins.
MedLine Citation:
PMID:  9002670     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The breast cancer susceptibility gene BRCA2 encodes a protein of 3418 amino acids which does not exhibit substantial sequence similarity to any other protein in the public databases. A dot matrix comparison of BRCA2 with itself revealed an eight times repeated motif in the segment of the protein encoded by exon 11. As a preliminary test of the hypothesis that these motifs are functionally significant, we have sequenced exon 11 of BRCA2 in six mammals. An alignment of the predicted protein sequences shows that, overall, the motifs have been conserved while much of the intervening sequences has diverged. These data support the notion that the BRC motifs are important in BRCA2 function. There is, however, considerable interspecies variation within certain motif units, raising the possibility of redundancy and that not all of the repeats are required for the normal function of BRCA2.
Authors:
G Bignell; G Micklem; M R Stratton; A Ashworth; R Wooster
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human molecular genetics     Volume:  6     ISSN:  0964-6906     ISO Abbreviation:  Hum. Mol. Genet.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-03-26     Completed Date:  1997-03-26     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  9208958     Medline TA:  Hum Mol Genet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  53-8     Citation Subset:  IM    
Affiliation:
Section of Molecular Carcinogenesis, Institute of Cancer Research, Sutton, UK.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/Z75664;  Z75665;  Z75666;  Z75667
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
BRCA2 Protein
Base Sequence
Conserved Sequence*
Cricetinae
DNA
Dogs
Exons*
Haplorhini
Humans
Mammals
Mice
Molecular Sequence Data
Neoplasm Proteins / genetics*
Repetitive Sequences, Nucleic Acid*
Sequence Homology, Amino Acid
Swine
Transcription Factors / genetics*
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/BRCA2 Protein; 0/Neoplasm Proteins; 0/Transcription Factors; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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