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BRAF Test and Cytological Diagnosis with a Single Fine Needle Cytology Sample.
MedLine Citation:
PMID:  23860494     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Objective: Recently, fine needle cytology (FNC) of the thyroid has been combined with biomolecular analysis. In particular, there has been detailed study of the V600E-BRAF mutation. The aim of our study is to demonstrate that with a single thyroid sample it is possible to obtain enough cellular material for both cytological diagnosis and a V600E-BRAF molecular test. Study Design: FNC was carried out under ultrasound guidance aided by an echographist and cytopathologist. We acquired one biopsy for each nodule with a 23-gauge needle without suction. The preparations were smeared by the pathologist onto one glass slide, air dried and stained with Diff-Quick. Cell adequacy was evaluated for each patient. The needle was washed by aspirating 2 ml of physiologic solution which was collected into a tube. The material was collected for molecular testing. Results: The following cytological diagnoses were made: not neoplastic, Tir2 (n = 227); indeterminate, Tir3 (n = 15); suspicious, Tir4 (n = 4), and malignancy, Tir5 (n = 12). The V600E-BRAF mutation was found in 0 of 227 Tir2 specimens, 2 of 15 (13.3%) Tir3 specimens, 2 of 4 (50%) Tir4 specimens and 9 of 12 (75%) Tir5 specimens. Conclusions: Our data showed that, in a routine clinical setting, FNC specimens can be handled properly to provide both morphological and molecular information. In fact, our tests show that with a single specimen it is possible to set up a slide for the cytological diagnosis and to obtain enough residual cellular material for DNA extraction (>70 ng) and for the identification of the V600E-BRAF mutation.
Authors:
Giuseppe Di Benedetto; Alessio Fabozzi; Carmine Rinaldi; Ciro Roberto Rinaldi
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-7-12
Journal Detail:
Title:  Acta cytologica     Volume:  57     ISSN:  0001-5547     ISO Abbreviation:  Acta Cytol.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-7-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370307     Medline TA:  Acta Cytol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  337-340     Citation Subset:  -    
Copyright Information:
Copyright © 2013 S. Karger AG, Basel.
Affiliation:
Cytopathology Service, Department of Clinical Pathology, ASL Caserta, Caserta, Italy.
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