Document Detail


BORIS, brother of the regulator of imprinted sites, is aberrantly expressed in hepatocellular carcinoma.
MedLine Citation:
PMID:  23237599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The brother of the regulator of imprinted sites (BORIS) is a novel member of the cancer testis antigen gene family, which are normally expressed only in spermatocytes, but abnormally activated in different malignancies.
AIM: The aim of this study was to explore the expression of BORIS in hepatocellular carcinoma (HCC) and its correlation with the clinicopathologic features and prognosis of HCC.
METHODS: We investigated BORIS expression in HCC cell lines and 105 primary HCC clinical surgical specimens using real-time polymerase chain reaction and Western blot analysis. We further examined the correlation of BORIS with a liver stem cell marker (CD90) in HCC tissues by histochemical double staining. The correlation of BORIS with clinicopathologic features and prognosis of HCC was analyzed using patient data.
RESULTS: The expression of BORIS was found in SMMC-7721, BEL-7402, and Huh-7, but not in hep-G2 cells. The expression rate of BORIS was significantly higher in the HCC tissues than in the adjacent noncancerous tissues (p=0.000). BORIS expression was correlated with the tumor size (p=0.000), CD90 expression (p=0.000), and satellite nodule (p=0.000). Kaplan-Meier survival curves showed that patients with positive expression of BORIS had lower overall survival rate (p=0.003).
CONCLUSIONS: Our data indicate that BORIS may be an auxiliary diagnosis index and a novel favorable prognostic indicator of HCC.
Authors:
Kefei Chen; Wenqing Huang; Bin Huang; YongGang Wei; Bo Li; Yan Ge; Yang Qin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-12-13
Journal Detail:
Title:  Genetic testing and molecular biomarkers     Volume:  17     ISSN:  1945-0257     ISO Abbreviation:  Genet Test Mol Biomarkers     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-22     Completed Date:  2013-06-24     Revised Date:  2014-02-04    
Medline Journal Info:
Nlm Unique ID:  101494210     Medline TA:  Genet Test Mol Biomarkers     Country:  United States    
Other Details:
Languages:  eng     Pagination:  160-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Carcinoma, Hepatocellular / genetics,  metabolism*,  mortality,  pathology*
Cardiopulmonary Resuscitation
Cell Line, Tumor
DNA-Binding Proteins / genetics*,  metabolism
Female
Gene Expression Regulation, Neoplastic
Hep G2 Cells
Humans
Kaplan-Meier Estimate
Liver Neoplasms / genetics,  metabolism*,  mortality,  pathology*
Low Density Lipoprotein Receptor-Related Protein-1 / genetics,  metabolism*
Male
Middle Aged
Polymerase Chain Reaction
Prognosis
Survival Rate
Chemical
Reg. No./Substance:
0/CTCFL protein, human; 0/DNA-Binding Proteins; 0/LRP1 protein, human; 0/Low Density Lipoprotein Receptor-Related Protein-1
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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