Document Detail


BNIPL-2 promotes the invasion and metastasis of human hepatocellular carcinoma cells.
MedLine Citation:
PMID:  17273740     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Enhanced cell migration and invasion play key roles in cancer metastasis. However, the molecules involved in this process are not fully understood. In this study, a full-length human BNIPL-2 (Bcl-2/adenovirus E1B 19 kDa interacting protein 2 like-2) cDNA was transfected into human hepatocellular carcinoma cells with low metastatic potential (MHCC97-L). The in vitro and in vivo effects of BNIPL-2 on cell invasion and metastasis were examined. In vitro analysis showed that the overexpression of BNIPL-2 increases cell invasion and promotes cell migration. The rates of intrahepatic and pulmonary metastasis in nude mice were also increased. Cdc42 activation assays and immunoblot analysis indicated that the activation of Cdc42 and the upregulation of CD44 were involved in the metastasis of cancer cells. The overexpression of BNIPL-2 promotes the invasion and metastasis of MHCC97-L cells. Thus, BNIPL-2 is a gene related with cancer metastasis.
Authors:
Li Xie; Wenxin Qin; Jinjun Li; Xianghuo He; Haitao Zhang; Genfu Yao; Huiqun Shu; Ming Yao; Dafang Wan; Jianren Gu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  17     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-02     Completed Date:  2007-05-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  605-10     Citation Subset:  IM    
Affiliation:
National Laboratory for Oncogenes and Related Genes, WHO Collaborating Center for Research on Cancer, Shanghai Cancer Institute, Shanghai Jiao Tong University, Shanghai 200032, P.R. China.
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MeSH Terms
Descriptor/Qualifier:
Adaptor Proteins, Signal Transducing / biosynthesis*
Animals
Carcinoma, Hepatocellular / metabolism,  pathology*
Cell Line, Tumor
Humans
Immunoblotting
Liver Neoplasms / metabolism,  pathology*
Mice
Mice, Nude
Neoplasm Invasiveness*
Neoplasm Metastasis*
Neoplasms, Experimental / pathology
Transfection
Chemical
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/BNIPL protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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