|BMP-7-induced cell cycle arrest of anaplastic thyroid carcinoma cells via p21(CIP1) and p27(KIP1).|
|PMID: 11453659 Owner: NLM Status: MEDLINE|
|The aim of the present study was to investigate the effect of bone morphogenetic protein (BMP-7) on thyroid carcinoma cell growth. Addition of BMP-7 inhibited the proliferation of four out of six human anaplastic thyroid carcinoma cell lines, observed as decreased incorporation of (3)H-thymidine and decreased cell number. The growth inhibitory effect was cell density-dependent; sparse cells were inhibited by BMP-7 whereas dense cells were not. Cell cycle analysis by flow cytometry showed an increased fraction of cells in the G1-phase and subsequent decrease in both S- and G2/M-phase after BMP-7 stimulation. Furthermore, BMP-7 caused an upregulation of the cyclin-dependent kinase inhibitors (CDKIs) p21 and p27, decreased activity of Cdk2 and Cdk6, and hypophosphorylation of the retinoblastoma protein (pRb). These findings suggest a growth inhibitory effect of BMP-7 on anaplastic thyroid carcinoma cells by inhibition of Cdk activity shifting the Rb protein to the hypophosphorylated state.|
|Franzén A; N E Heldin|
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|Type: Journal Article; Research Support, Non-U.S. Gov't|
|Title: Biochemical and biophysical research communications Volume: 285 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2001 Jul|
|Created Date: 2001-07-16 Completed Date: 2001-08-16 Revised Date: 2012-06-25|
Medline Journal Info:
|Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States|
|Languages: eng Pagination: 773-81 Citation Subset: IM|
|Copyright 2001 Academic Press.|
|Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University Hospital, SE-751 85 Uppsala, Sweden. Asa.Franzen@genpat.uu.se|
|APA/MLA Format Download EndNote Download BibTex|
Bone Morphogenetic Protein 7
Bone Morphogenetic Proteins / pharmacology*
Carcinoma / metabolism*, pathology
Cell Cycle / drug effects
Cell Cycle Proteins / metabolism*
Cell Division / drug effects
Cyclin-Dependent Kinase 2
Cyclin-Dependent Kinase 4
Cyclin-Dependent Kinase 6
Cyclin-Dependent Kinase Inhibitor p21
Cyclin-Dependent Kinase Inhibitor p27
Cyclin-Dependent Kinases / metabolism
Cyclins / metabolism*
Dose-Response Relationship, Drug
G1 Phase / drug effects
Phosphorylation / drug effects
Protein-Serine-Threonine Kinases / metabolism
Retinoblastoma Protein / metabolism
Thyroid Neoplasms / metabolism*, pathology
Transforming Growth Factor beta*
Tumor Cells, Cultured
Tumor Suppressor Proteins*
Up-Regulation / drug effects
|0/BMP7 protein, human; 0/Bone Morphogenetic Protein 7; 0/Bone Morphogenetic Proteins; 0/CDKN1A protein, human; 0/Cell Cycle Proteins; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Proto-Oncogene Proteins; 0/Retinoblastoma Protein; 0/Transforming Growth Factor beta; 0/Tumor Suppressor Proteins; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; EC 126.96.36.199/Protein-Serine-Threonine Kinases; EC 188.8.131.52/CDC2-CDC28 Kinases; EC 184.108.40.206/CDK2 protein, human; EC 220.127.116.11/CDK4 protein, human; EC 18.104.22.168/CDK6 protein, human; EC 22.214.171.124/Cyclin-Dependent Kinase 2; EC 126.96.36.199/Cyclin-Dependent Kinase 4; EC 188.8.131.52/Cyclin-Dependent Kinase 6; EC 184.108.40.206/Cyclin-Dependent Kinases|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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