| BMP-7-induced cell cycle arrest of anaplastic thyroid carcinoma cells via p21(CIP1) and p27(KIP1). | |
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MedLine Citation:
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PMID: 11453659 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the present study was to investigate the effect of bone morphogenetic protein (BMP-7) on thyroid carcinoma cell growth. Addition of BMP-7 inhibited the proliferation of four out of six human anaplastic thyroid carcinoma cell lines, observed as decreased incorporation of (3)H-thymidine and decreased cell number. The growth inhibitory effect was cell density-dependent; sparse cells were inhibited by BMP-7 whereas dense cells were not. Cell cycle analysis by flow cytometry showed an increased fraction of cells in the G1-phase and subsequent decrease in both S- and G2/M-phase after BMP-7 stimulation. Furthermore, BMP-7 caused an upregulation of the cyclin-dependent kinase inhibitors (CDKIs) p21 and p27, decreased activity of Cdk2 and Cdk6, and hypophosphorylation of the retinoblastoma protein (pRb). These findings suggest a growth inhibitory effect of BMP-7 on anaplastic thyroid carcinoma cells by inhibition of Cdk activity shifting the Rb protein to the hypophosphorylated state. |
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Authors:
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Franzén A; N E Heldin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 285 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2001 Jul |
Date Detail:
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Created Date: 2001-07-16 Completed Date: 2001-08-16 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 773-81 Citation Subset: IM |
Copyright Information:
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Copyright 2001 Academic Press. |
Affiliation:
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Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University Hospital, SE-751 85 Uppsala, Sweden. Asa.Franzen@genpat.uu.se |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bone Morphogenetic Protein 7 Bone Morphogenetic Proteins / pharmacology* CDC2-CDC28 Kinases* Carcinoma / metabolism*, pathology Cell Count Cell Cycle / drug effects Cell Cycle Proteins / metabolism* Cell Division / drug effects Cyclin-Dependent Kinase 2 Cyclin-Dependent Kinase 4 Cyclin-Dependent Kinase 6 Cyclin-Dependent Kinase Inhibitor p21 Cyclin-Dependent Kinase Inhibitor p27 Cyclin-Dependent Kinases / metabolism Cyclins / metabolism* Dose-Response Relationship, Drug Flow Cytometry G1 Phase / drug effects Humans Phosphorylation / drug effects Protein-Serine-Threonine Kinases / metabolism Proto-Oncogene Proteins* Retinoblastoma Protein / metabolism Thyroid Neoplasms / metabolism*, pathology Transforming Growth Factor beta* Tumor Cells, Cultured Tumor Suppressor Proteins* Up-Regulation / drug effects |
| Chemical | |
Reg. No./Substance:
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0/BMP7 protein, human; 0/Bone Morphogenetic Protein 7; 0/Bone Morphogenetic Proteins; 0/CDKN1A protein, human; 0/Cell Cycle Proteins; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Proto-Oncogene Proteins; 0/Retinoblastoma Protein; 0/Transforming Growth Factor beta; 0/Tumor Suppressor Proteins; 147604-94-2/Cyclin-Dependent Kinase Inhibitor p27; EC 2.7.1.37/CDK2 protein, human; EC 2.7.1.37/CDK4 protein, human; EC 2.7.1.37/CDK6 protein, human; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.22/CDC2-CDC28 Kinases; EC 2.7.11.22/Cyclin-Dependent Kinase 2; EC 2.7.11.22/Cyclin-Dependent Kinase 4; EC 2.7.11.22/Cyclin-Dependent Kinase 6; EC 2.7.11.22/Cyclin-Dependent Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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