Document Detail


BMP-4 regulates the dorsal-ventral differences in FGF/MAPKK-mediated mesoderm induction in Xenopus.
MedLine Citation:
PMID:  7589804     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent studies on Xenopus development have revealed an increasingly complex array of inductive, prepatterning, and competence signals that are necessary for proper mesoderm formation. In this study, we establish that fibroblast growth factor (FGF) signals through mitogen-activated protein kinase kinase (MAPKK) to induce mesodermal gene expression. We demonstrate that a partially activated form of MAPKK restores expression of the mesodermal genes Xcad-3 and Xbra, eliminated by the dominant-negative FGF receptor (delta FGFR). Similar to the results reported earlier with delta FGFR, expression of a dominant-negative form of MAPKK (MAPKKD) preferentially eliminates the dorsal expression of Xcad-3 and Xbra. We tested whether the regional localization of bone morphogenetic protein-4 (BMP-4) could explain why both MAPKKD and delta FGFR eliminate the dorsal and not the ventral expression of Xcad-3 and Xbra. We show that ectopic expression of BMP-4 is sufficient to maintain the dorsal expression of Xcad-3 and Xbra in embryos containing delta FGFR and that expression of a dominant-negative BMP receptor reduces the dorsal-ventral differences in delta FGFR embryos. These results indicate that regional localization of BMP-4 is responsible for the dorsal-ventral asymmetry in FGF/MAPKK-mediated mesoderm induction.
Authors:
J Northrop; A Woods; R Seger; A Suzuki; N Ueno; E Krebs; D Kimelman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  172     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1995-12-26     Completed Date:  1995-12-26     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  242-52     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, School of Medicine, University of Washington, Seattle 98195-7350, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Morphogenetic Proteins
DNA-Binding Proteins / biosynthesis
Embryo, Nonmammalian / cytology,  physiology*
Female
Fetal Proteins / biosynthesis*
Gene Expression*
Growth Substances / biosynthesis
In Situ Hybridization
Male
Mesoderm / cytology,  physiology*
Mitogen-Activated Protein Kinase Kinases
Mutagenesis
Protein Biosynthesis*
Protein Kinases / biosynthesis,  genetics,  metabolism*
Proteins / physiology
Receptors, Fibroblast Growth Factor / biosynthesis*,  physiology
Signal Transduction
T-Box Domain Proteins*
Transcription, Genetic
Xenopus / embryology*
Xenopus Proteins*
Grant Support
ID/Acronym/Agency:
DK 42528/DK/NIDDK NIH HHS; GM 42508/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bone Morphogenetic Proteins; 0/Brachyury protein; 0/DNA-Binding Proteins; 0/Fetal Proteins; 0/Growth Substances; 0/Proteins; 0/Receptors, Fibroblast Growth Factor; 0/T-Box Domain Proteins; 0/Xcad-3 protein, Xenopus; 0/Xenopus Proteins; EC 2.7.-/Protein Kinases; EC 2.7.12.2/Mitogen-Activated Protein Kinase Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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