|BM88 is an early marker of proliferating precursor cells that will differentiate into the neuronal lineage.|
|PMID: 15548196 Owner: NLM Status: MEDLINE|
|Progression of progenitor cells towards neuronal differentiation is tightly linked with cell cycle control and the switch from proliferative to neuron-generating divisions. We have previously shown that the neuronal protein BM88 drives neuroblastoma cells towards exit from the cell cycle and differentiation into a neuronal phenotype in vitro. Here, we explored the role of BM88 during neuronal birth, cell cycle exit and the initiation of differentiation in vivo. By double- and triple-labelling with the S-phase marker BrdU or the late G2 and M-phase marker cyclin B1, antibodies to BM88 and markers of the neuronal or glial cell lineages, we demonstrate that in the rodent forebrain, BM88 is expressed in multipotential progenitor cells before terminal mitosis and in their neuronal progeny during the neurogenic interval, as well as in the adult. Further, we defined at E16 a cohort of proliferative progenitors that exit S phase in synchrony, and by following their fate for 24 h we show that BM88 is associated with the dynamics of neuron-generating divisions. Expression of BM88 was also evident in cycling cortical radial glial cells, which constitute the main neurogenic population in the cerebral cortex. In agreement, BM88 expression was markedly reduced and restricted to a smaller percentage of cells in the cerebral cortex of the Small eye mutant mice, which lack functional Pax6 and exhibit severe neurogenesis defects. Our data show an interesting correlation between BM88 expression and the progression of progenitor cells towards neuronal differentiation during the neurogenic interval.|
|Yassemi Koutmani; Catherine Hurel; Evangelia Patsavoudi; Michael Hack; Magdalena Gotz; Dimitra Thomaidou; Rebecca Matsas|
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|Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't|
|Title: The European journal of neuroscience Volume: 20 ISSN: 0953-816X ISO Abbreviation: Eur. J. Neurosci. Publication Date: 2004 Nov|
|Created Date: 2004-11-19 Completed Date: 2005-03-01 Revised Date: 2009-11-19|
Medline Journal Info:
|Nlm Unique ID: 8918110 Medline TA: Eur J Neurosci Country: France|
|Languages: eng Pagination: 2509-23 Citation Subset: IM|
|Laboratory of Cellular and Molecular Neurobiology, Hellenic Pasteur Institute, 127 Vassilissis Sofias Avenue, Athens 115 21, Greece.|
|APA/MLA Format Download EndNote Download BibTex|
Amino Acid Transport System X-AG / metabolism
Antigens / metabolism
Biological Markers / metabolism
Blotting, Western / methods
Brain / cytology, embryology, growth & development, metabolism
Bromodeoxyuridine / metabolism
Carbocyanines / metabolism
Cell Count / methods
Cell Cycle / physiology
Cell Differentiation / physiology*
Cell Lineage / physiology*
Cyclin B / metabolism
Gene Expression Regulation, Developmental / physiology
Glial Fibrillary Acidic Protein / metabolism
Homeodomain Proteins / genetics, metabolism
Immunohistochemistry / methods
Intermediate Filament Proteins / metabolism
Membrane Proteins / metabolism
Mice, Inbred C57BL
Mice, Neurologic Mutants
Nerve Tissue Proteins / metabolism
Neural Cell Adhesion Molecule L1 / metabolism
Neural Networks (Computer)
Neurons / cytology, metabolism, physiology*
Paired Box Transcription Factors
Phosphopyruvate Hydratase / metabolism
Proteoglycans / metabolism
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction / methods
Sialic Acids / metabolism
Stem Cells / cytology*, physiology
Thymidine / pharmacokinetics
Tritium / pharmacokinetics
|0/3,3'-dihexadecylindocarbocyanine; 0/Amino Acid Transport System X-AG; 0/Antigens; 0/BM88 protein, mouse; 0/BM88 protein, rat; 0/Biological Markers; 0/Carbocyanines; 0/Ccnb1 protein, mouse; 0/Ccnb1 protein, rat; 0/Cyclin B; 0/Cyclin B1; 0/Eye Proteins; 0/Glial Fibrillary Acidic Protein; 0/Homeodomain Proteins; 0/Intermediate Filament Proteins; 0/Membrane Proteins; 0/Nerve Tissue Proteins; 0/Neural Cell Adhesion Molecule L1; 0/PAX6 protein; 0/Paired Box Transcription Factors; 0/Proteoglycans; 0/RNA, Messenger; 0/Repressor Proteins; 0/Sialic Acids; 0/chondroitin sulfate proteoglycan 4; 0/nestin; 0/polysialyl neural cell adhesion molecule; 10028-17-8/Tritium; 50-89-5/Thymidine; 59-14-3/Bromodeoxyuridine; EC 22.214.171.124/Phosphopyruvate Hydratase|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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