| BLIMP1 regulates cell growth through repression of p53 transcription. | |
| | |
MedLine Citation:
|
PMID: 17264218 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Tight regulation of p53 is essential for maintaining normal cell growth. Here we report that BLIMP1 acts in an autoregulatory feedback loop that controls p53 activity through repression of p53 transcription. p53 binds to and positively regulates BLIMP1, which encodes for a known B cell transcriptional repressor. Knockdown of BLIMP1 by siRNA results in both apoptosis and growth arrest in human colon cancer cells and cell-cycle arrest in primary human fibroblasts. Interestingly, the levels of both p53 mRNA and protein are substantially increased after BLIMP1 depletion, which is accompanied by the induction of p53 target genes. Importantly, the apoptosis induced by BLIMP1 depletion in HCT116 cells is largely abrogated in cells lacking p53 or in cells depleted in p53 by siRNA. We further demonstrate that BLIMP1 binds to the p53 promoter and represses p53 transcription, and this provides a mechanistic explanation for the induction of p53 response in cells depleted of BLIMP1. Hence, suppression of p53 transcription is a crucial function of endogenous BLIMP1 and is essential for normal cell growth. |
| | |
Authors:
|
Junli Yan; Jianming Jiang; Ching Aeng Lim; Qiang Wu; Huck-Hui Ng; Keh-Chuang Chin |
Related Documents
:
|
12485438 - Cell cycle deregulation and xeroderma pigmentosum group c cell transformation. 7784088 - Gamma-radiation-induced apoptosis in human colorectal adenoma and carcinoma cell lines ... 19771278 - Cell cycle regulatory protein expression profiles by adenovirus p53 infection in human ... 6354748 - Reduction in p53 synthesis during differentiation of friend-erythroleukemia cells. corr... 11337268 - Vascular invasion in squamous cell carcinomas of human oral mucosa. 17994898 - Noninvasive measurement of three-dimensional morphology of adhered animal cells employi... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-30 |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 104 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2007 Feb |
Date Detail:
|
Created Date: 2007-02-07 Completed Date: 2007-03-22 Revised Date: 2013-06-06 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
|
Languages: eng Pagination: 1841-6 Citation Subset: IM |
Affiliation:
|
Gene Expression Laboratory, Genome Institute of Singapore, Singapore. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Cell Enlargement* Cell Line Cell Survival / genetics Gene Expression Regulation / physiology* HCT116 Cells Humans Protein Binding / genetics Repressor Proteins / metabolism, physiology* Transcription Factors / metabolism, physiology* Transcription, Genetic* Tumor Suppressor Protein p53 / antagonists & inhibitors*, biosynthesis, genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Repressor Proteins; 0/Transcription Factors; 0/Tumor Suppressor Protein p53; 138415-26-6/PRDM1 protein, human |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A gene cluster encoding cholesterol catabolism in a soil actinomycete provides insight into Mycobact...
Next Document: A single 1-h bout of evening exercise increases basal FFA flux without affecting VLDL-triglyceride a...