| BK virus (BKV) quantification in urine samples of bone marrow transplanted patients is helpful for diagnosis of hemorrhagic cystitis, although wide individual variations exist. | |
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MedLine Citation:
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PMID: 12589836 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Hemorrhagic cystitis (HC) in allogeneic bone marrow transplanted (BMT) patients is associated with BK virus (BKV) reactivation manifested as BK viruria. However, since 77-90% of all adult BMT patients excrete BKV, viral reactivation alone cannot be responsible for HC. Recently, a significant overrepresentation of C-->G mutations in the Sp1 binding site in the non-coding control region (NCCR) of BKV was shown to be present in HC patients and absent in non-HC patients. OBJECTIVES: We aimed to investigate if this mutation resulted in excessive BKV excretion in HC patients. STUDY DESIGN: A Real-Time PCR was developed and used to quantify BKV in urine samples from 21 patients with HC, with and without the mutations, as well as from patients without HC. RESULTS: Quantification of BKV was successful in 18 of 21 urine patients (six with and six without C-->G mutations) and six patients without HC. A mean of 3.0 x 10(6) BKV copies/microl was detected in urine samples of HC patients with C-->G mutations, compared to a mean of 1.5 x 10(6) BKV copies/microl in HC patients without C-->G mutations and a mean of 1.0 x 10(6) BKV copies/microl in patients without HC. The obtained differences were however not statistically significant, due to one individual non-HC patient with an extremely high BKV copy number. Nevertheless, while 50% of the samples in the HC groups expressed 1 x 10(6) copies/microl or more, only one of the samples in the non-HC group contained a virus quantity higher than 5 x 10(5) copies. CONCLUSIONS: Although we could not confirm that the C-->G mutations in the Sp1 site of BKV were responsible for an increased viral load in patients with HC, our data suggest that levels of BKV above 10(4) copies/microl may indicate a risk for HC. |
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Authors:
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Peter Priftakis; Gordana Bogdanovic; Parviz Kokhaei; Håkan Mellstedt; Tina Dalianis |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology Volume: 26 ISSN: 1386-6532 ISO Abbreviation: J. Clin. Virol. Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2003-02-18 Completed Date: 2003-04-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9815671 Medline TA: J Clin Virol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 71-7 Citation Subset: IM |
Affiliation:
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Department of Oncology-Pathology, Karolinska Institute, Cancer Center Karolinska, CCK R8:01, 171 76 Stockholm, Sweden. peter.priftakis@cck.ki.se |
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| MeSH Terms | |
Descriptor/Qualifier:
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BK Virus
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genetics,
isolation & purification* Binding Sites / genetics Bone Marrow Transplantation* Computer Systems Cystitis / diagnosis, virology* DNA Mutational Analysis DNA, Viral / urine* Hemorrhage / virology Humans Immunocompromised Host Point Mutation Polymerase Chain Reaction Polyomavirus Infections / diagnosis, urine*, virology Sp1 Transcription Factor / metabolism Transplantation, Homologous Tumor Virus Infections / diagnosis, urine*, virology Urine / virology Virus Activation |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/Sp1 Transcription Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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