Document Detail


BK polyomavirus with archetypal and rearranged non-coding control regions are present in cerebrospinal fluids from patients with neurological complications.
MedLine Citation:
PMID:  22552944     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BK polyomavirus (BKPyV) has been recently postulated as an emerging opportunistic pathogen of the human central nervous system (CNS), but it is not known whether specific strains are associated with the neurotropic character of BKPyV. The presence of BKPyV large T-antigen DNA was examined in 2,406 cerebrospinal fluid (CSF) samples from neurological patients with suspected JC polyomavirus infection. Twenty patients had a large T-antigen DNA positive specimen. The non-coding control region (NCCR) of the BKPyV strains amplified from CSF from these 20 patients and from strains circulating in renal and bone marrow transplant recipients and from healthy pregnant women was sequenced. The archetypal conformation was the most prevalent in all groups and 14 of the neurological patients harboured archetypal strains, while the six remaining patients possessed BKPyV with rearranged NCCR similar to previously reported variants from non-neurological patients. Transfection studies in Vero cells revealed that 5 out of 6 early and 4 out of 6 late rearranged promoters of these CSF isolates showed significantly higher activity than the corresponding archetypal promoter. From seven of the neurological patients with BKPyV DNA positive CSF, paired serum samples were available. Five of them were negative for BKPyV DNA, while serum from the remaining two patients harbored BKPyV strains with archetypal NCCR that differed from those present in their CSF. Our results suggest that NCCR rearrangements are not a hallmark for BKPyV neurotropism and the dissemination of a rearranged NCCR from the blood may not be the origin of BKPyV CNS infection.
Authors:
Ana Bárcena-Panero; Juan E Echevarria; Marijke Van Ghelue; Giovanni Fedele; Enrique Royuela; Nancy Gerits; Ugo Moens
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-2
Journal Detail:
Title:  The Journal of general virology     Volume:  -     ISSN:  1465-2099     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
National Microbiology Centre, Carlos III Health Institute, Majadahonda, Madrid, Spain;
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