Document Detail


BIOTECON diagnostics foodproof Listeria monocytogenes Detection Kit, 5' nuclease in combination with the foodproof ShortPrep II Kit.
MedLine Citation:
PMID:  22468346     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
A method was developed for the detection of Listeria monocytogenes in food. The method is based on real-time PCR using hydrolysis probes (5' Nuclease). This advanced PCR method was designed to reduce the time necessary to achieve results from PCR reactions and to enable the user to monitor the amplification of the PCR product simultaneously, in real-time. After DNA isolation using the BIOTECON foodproof ShortPrep II Kit designed for the rapid preparation of L. monocytogenes DNA for direct use in PCR, the real-time detection of L. monocytogenes DNA is carried out using the foodproof Listeria monocytogenes Detection Kit. The kit provides primers and hydrolysis probes for sequence-specific detection, convenient premixed reagents, and controls for reliable interpretation of results. For the internal comparison study, three different foods (soft cheese, coalfish, and smoked ham) were analyzed, chosen from the 15 food groups recommended by the AOAC Research Institute for detection of L. monocytogenes. From each food, 20 samples were inoculated with a low level (1-10 CFU/25 g) and 20 samples with a high level (10-50 CFU/25 g) of L. monocytogenes. Additionally, five nonspiked samples were prepared from each food. Depending on the matrix, the food samples were examined with the test kits and compared with the cultural methods according to the U.S. Food and Drug Administration's Bacteriological Analytical Manual or the U.S. Department of Agriculture/Food Safety and Inspection Service Microbiology Laboratory Guidebook.
Authors:
Benjamin Junge; Cordt Grönewald; Kornelia Berghof-Jäger
Related Documents :
16111136 - The impact of wet flue gas desulfurization scrubbing on mercury emissions from coal-fir...
16441916 - Absorption, tissue distribution and excretion of pelargonidin and its metabolites follo...
15752496 - Accumulation of mercury in the tissues of the common octopus octopus vulgaris (l.) in t...
1812026 - Dietary intake of pesticide residues: cadmium, mercury, and lead.
1158536 - Assessment of the world food situation-present and future.
6689516 - The potential value of cultured dairy products for child nutrition.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of AOAC International     Volume:  95     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2012 Jan-Feb
Date Detail:
Created Date:  2012-04-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  92-9     Citation Subset:  IM    
Affiliation:
BIOTECON Diagnostics GmbH, Hermannswerder Haus 17, 14473 Potsdam, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Quantitative HPLC method for determining two of the major active phthalides from Ligusticum porteri ...
Next Document:  Detection of Salmonella in meat: comparative and interlaboratory validation of a noncomplex and cost...