Document Detail


BIOTECON diagnostics foodproof E. coli O157 detection kit, 5' nuclease for E. coli O157 in combination with foodproof ShortPrep II Kit.
MedLine Citation:
PMID:  22320092     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
The method describes the detection of Escherichia coli O157 in food. The method is based on real-time PCR using hydrolysis probes (5' Nuclease). This advanced PCR method was designed to reduce the time necessary to achieve results from PCR reactions and enable the user to monitor the amplification of the PCR product simultaneously in real time. After DNA isolation using the BIOTECON foodproof ShortPrep II Kit designed for the rapid preparation of E. coli O157 DNA for direct use in PCR, the real-time detection of E. coli O157 DNA is carried out using the foodproof E. coli O157 Detection Kit. The kit provides primers and hydrolysis probes for sequence-specific detection, convenient premixed reagents, and controls for reliable interpretation of results. For repeatability studies three different foods (egg salad, large bockwurst/frankfurter, and apple juice) were analyzed, chosen from the 15 food groups recommended by the AOAC Research Institute for E. coli O157 detection. From each food, 20 samples were inoculated with a low level (1-10 CFU/25 g) and 20 samples with a high level (10-50 CFU/25 g) ofE. coli O157. Additionally, five nonspiked samples were prepared from each food. Depending on the matrix, the food samples were examined with the test kits and compared with the cultural methods according to the U.S. Food and Drug Administration Bacteriological Analytical Manual or the U.S. Department of Agriculture/Food Safety and Inspection Service Microbiology Laboratory Guidebook.
Authors:
Benjamin Junge; Cordt Grönewald; Kornelia Berghof-Jäger
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  94     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2011 Nov-Dec
Date Detail:
Created Date:  2012-02-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1846-52     Citation Subset:  IM    
Affiliation:
BIOTECON Diagnostics GmbH, Hermannswerder Haus 17, 14473 Potsdam, Germany. bjunge@bc-diagnostics.com
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