Document Detail

BHK cells transfected with NCX3 are more resistant to hypoxia followed by reoxygenation than those transfected with NCX1 and NCX2: Possible relationship with mitochondrial membrane potential.
MedLine Citation:
PMID:  17343909     Owner:  NLM     Status:  MEDLINE    
The specific role played by NCX1, NCX2, and NCX3, the three isoforms of the Na+/Ca2+ exchanger (NCX), has been explored during hypoxic conditions in BHK cells stably transfected with each of these isoforms. Six major findings emerged from the present study: (1) all the three isoforms were highly expressed on the plasma membranes of BHK cells; (2) under physiological conditions, the three NCX isoforms showed similar functional activity; (3) hypoxia plus reoxygenation induced a lower increase of [Ca2+]i in BHK-NCX3-transfected cells than in BHK-NCX1- and BHK-NCX2-transfected cells; (4) NCX3-transfected cells were more resistant to chemical hypoxia plus reoxygenation than NCX1- and NCX2-transfected cells. Interestingly, such augmented resistance was eliminated by CBDMD (10 microM), an inhibitor of NCX and by the specific silencing of the NCX3 isoform; (5) chemical hypoxia plus reoxygenation produced a loss of mitochondrial membrane potential in NCX1- and NCX2-transfected cells, but not in NCX3-transfected cells; (6) the forward mode of operation in NCX3-transfected cells was not affected by ATP depletion, as it occurred in NCX1- and NCX2-transfected cells. Altogether, these results indicate that the brain specifically expressed NCX3 isoform more significantly contributes to the maintenance of [Ca2+]i homeostasis during experimental conditions mimicking ischemia, thereby preventing mitochondrial delta psi collapses and cell death.
Agnese Secondo; Rosaria Ilaria Staiano; Antonella Scorziello; Rossana Sirabella; Francesca Boscia; Annagrazia Adornetto; Valeria Valsecchi; Pasquale Molinaro; Lorella Maria Teresa Canzoniero; Gianfranco Di Renzo; Lucio Annunziato
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-06
Journal Detail:
Title:  Cell calcium     Volume:  42     ISSN:  0143-4160     ISO Abbreviation:  Cell Calcium     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-10-02     Completed Date:  2008-02-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006226     Medline TA:  Cell Calcium     Country:  Scotland    
Other Details:
Languages:  eng     Pagination:  521-35     Citation Subset:  IM    
Division of Pharmacology, Department of Neuroscience, School of Medicine, University of Naples Federico II, Via Pansini 5, 80131 Naples, Italy.
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MeSH Terms
Adenosine Triphosphate / metabolism
Blotting, Western
Calcium / metabolism
Cell Hypoxia
Cell Line
Cell Survival / drug effects
Homeostasis / drug effects
Membrane Potential, Mitochondrial / drug effects,  physiology*
Oxygen / pharmacology*
Protein Isoforms / genetics,  physiology
RNA, Small Interfering / genetics
Sodium / metabolism
Sodium-Calcium Exchanger / genetics,  physiology*
Reg. No./Substance:
0/Protein Isoforms; 0/RNA, Small Interfering; 0/Sodium-Calcium Exchanger; 0/sodium-calcium exchanger 1; 56-65-5/Adenosine Triphosphate; 7440-23-5/Sodium; 7440-70-2/Calcium; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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