Document Detail


BDNF accelerates gene expression in cultured cerebellar granule neurons.
MedLine Citation:
PMID:  9541745     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study reports that in purified cultures of postnatal cerebellar granule cells, BDNF significantly accelerated GABAA receptor alpha 6 subunit (GABAA alpha 6) mRNA expression, a marker for terminally differentiated cerebellar granule neurons, and also accelerated p21cip1 expression. p21cip1 is a general cyclin-dependent kinase (Cdk) inhibitor that can inhibit progression through the cell cycle. Alternatively, the expression of p27kip1, another Cdk inhibitor closely related to p21cip1, is not modified by BDNF. In cultured granule cells, the increase in p21cip1 expression induced by BDNF occurred after dividing granule cells had left the cell cycle and thus was not required to direct granule neuron precursors out of the cell cycle. p21cip1 may have an alterative function during granule neuron terminal differentiation, separate from its ability to regulate cell cycle exit. This report shows that, in vitro, BDNF accelerates granule cell gene expression and may thus modulate cerebellar granule cell differentiation.
Authors:
X Lin; H Cui; R F Bulleit
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research. Developmental brain research     Volume:  105     ISSN:  0165-3806     ISO Abbreviation:  Brain Res. Dev. Brain Res.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-06-08     Completed Date:  1998-06-08     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8908639     Medline TA:  Brain Res Dev Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  277-86     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, University of Maryland School of Medicine, Baltimore 21201, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain-Derived Neurotrophic Factor / pharmacology*
Cell Count
Cell Differentiation / drug effects
Cells, Cultured
Cerebellum / cytology,  drug effects,  metabolism*
Cyclin-Dependent Kinases / metabolism
Cytoplasmic Granules / drug effects,  ultrastructure
DNA / biosynthesis
Gene Expression / drug effects,  physiology*
Immunoblotting
Mice
Neurons / drug effects*
RNA, Messenger / biosynthesis
Thymidine / metabolism
Chemical
Reg. No./Substance:
0/Brain-Derived Neurotrophic Factor; 0/RNA, Messenger; 50-89-5/Thymidine; 9007-49-2/DNA; EC 2.7.11.22/Cyclin-Dependent Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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