| BDNF accelerates gene expression in cultured cerebellar granule neurons. | |
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MedLine Citation:
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PMID: 9541745 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study reports that in purified cultures of postnatal cerebellar granule cells, BDNF significantly accelerated GABAA receptor alpha 6 subunit (GABAA alpha 6) mRNA expression, a marker for terminally differentiated cerebellar granule neurons, and also accelerated p21cip1 expression. p21cip1 is a general cyclin-dependent kinase (Cdk) inhibitor that can inhibit progression through the cell cycle. Alternatively, the expression of p27kip1, another Cdk inhibitor closely related to p21cip1, is not modified by BDNF. In cultured granule cells, the increase in p21cip1 expression induced by BDNF occurred after dividing granule cells had left the cell cycle and thus was not required to direct granule neuron precursors out of the cell cycle. p21cip1 may have an alterative function during granule neuron terminal differentiation, separate from its ability to regulate cell cycle exit. This report shows that, in vitro, BDNF accelerates granule cell gene expression and may thus modulate cerebellar granule cell differentiation. |
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Authors:
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X Lin; H Cui; R F Bulleit |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Brain research. Developmental brain research Volume: 105 ISSN: 0165-3806 ISO Abbreviation: Brain Res. Dev. Brain Res. Publication Date: 1998 Feb |
Date Detail:
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Created Date: 1998-06-08 Completed Date: 1998-06-08 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8908639 Medline TA: Brain Res Dev Brain Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 277-86 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, University of Maryland School of Medicine, Baltimore 21201, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain-Derived Neurotrophic Factor / pharmacology* Cell Count Cell Differentiation / drug effects Cells, Cultured Cerebellum / cytology, drug effects, metabolism* Cyclin-Dependent Kinases / metabolism Cytoplasmic Granules / drug effects, ultrastructure DNA / biosynthesis Gene Expression / drug effects, physiology* Immunoblotting Mice Neurons / drug effects* RNA, Messenger / biosynthesis Thymidine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Brain-Derived Neurotrophic Factor; 0/RNA, Messenger; 50-89-5/Thymidine; 9007-49-2/DNA; EC 2.7.11.22/Cyclin-Dependent Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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