| BDNF-induced changes in the expression of the translation machinery in hippocampal neurons: protein levels and dendritic mRNA. | |
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MedLine Citation:
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PMID: 19702335 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BDNF plays a key role in neuronal development, in short- and long-term changes in synaptic activity, and in neuronal survival. These effects are mediated, to a great extent, by changes in protein synthesis. We conducted a gel-based proteome profiling of the long-term (12 h) effects of BDNF in cultured hippocampal neurons. BDNF changed the abundance of proteins involved in (i) Nucleobase, nucleoside, nucleotide and nucleic acid metabolism, (ii) protein metabolism, (iii) carbohydrate metabolism, (iv) regulators of apoptosis, and (v) regulators of cell proliferation. A large majority of the identified proteins involved in translation activity were upregulated, but not all changes in the protein content were correlated with alterations in the corresponding mRNA. The upregulation of Seryl-aminoacyl-tRNA-synthetase and Eef2 was sensitive to the mTOR inhibitor rapamycin, as determined by Western blot. Since the mRNAs for proteins involved in translation represent a large fraction of the diversity of dendritic mRNAs, we investigated the effect of BDNF on the distribution of the transcripts in the soma versus neurite compartments. The increase in mRNA for proteins of the translation machinery in the soma was differentially coupled with the upregulation of neurite transcripts. BDNF also downregulated specific mRNAs in neurite compartments suggesting that the neurotrophin may act by regulating mRNA stability and thereby affecting the dendritic protein content. |
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Authors:
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Bruno Manadas; Ana Rita Santos; Krisztina Szabadfi; João R Gomes; Spiros D Garbis; Michael Fountoulakis; Carlos B Duarte |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of proteome research Volume: 8 ISSN: 1535-3907 ISO Abbreviation: J. Proteome Res. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-10-02 Completed Date: 2010-02-01 Revised Date: 2010-02-02 |
Medline Journal Info:
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Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: United States |
Other Details:
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Languages: eng Pagination: 4536-52 Citation Subset: IM |
Affiliation:
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Center for Neuroscience and Cell Biology, Department of Zoology, University of Coimbra, Coimbra, Portugal. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acyl-tRNA Synthetases
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metabolism Animals Brain-Derived Neurotrophic Factor / genetics, metabolism* Carrier Proteins / metabolism Cells, Cultured Cluster Analysis Dendrites / metabolism* Electrophoresis, Gel, Two-Dimensional Hippocampus / metabolism* Isotope Labeling Models, Biological Peptide Elongation Factors / metabolism Phosphotransferases (Alcohol Group Acceptor) / metabolism Protein Biosynthesis Proteome / metabolism* RNA, Messenger / metabolism* Rats |
| Chemical | |
Reg. No./Substance:
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0/Brain-Derived Neurotrophic Factor; 0/Carrier Proteins; 0/Peptide Elongation Factors; 0/Proteome; 0/RNA, Messenger; EC 2.7.1.-/Phosphotransferases (Alcohol Group Acceptor); EC 2.7.1.-/mTOR protein; EC 6.1.1.-/Amino Acyl-tRNA Synthetases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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