| BCR-induced superoxide negatively regulates B-cell proliferation and T-cell-independent type 2 Ab responses. | |
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MedLine Citation:
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PMID: 19877015 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Superoxide and its derivatives have been implicated as secondary messenger molecules that influence signaling cascades in non-phagocytes. B lymphocytes produce superoxide after BCR ligation. We found that these ROS regulate B-cell signaling and entry into the cell cycle. B cells from mice deficient in the gp91(phox) subunit of the NADPH oxidase complex are unable to generate ROS after BCR ligation. However, after BCR stimulation, more gp91(phox) KO B cells enter the G1 stage of the cell cycle and proliferate than WT B cells. BCR ligation leads to a more rapid decrease in p27(Kip1) levels in gp91(phox) KO B cells. Gp91(phox) KO mice display enhanced T-cell-independent type 2, but normal T-dependent Ab responses. ROS-dependent regulation of BCR-induced proliferation may help modulate the size of the humoral response to T-cell-independent type 2 Ag immunization. |
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Authors:
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Sabrina M Richards; Edward A Clark |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of immunology Volume: 39 ISSN: 1521-4141 ISO Abbreviation: Eur. J. Immunol. Publication Date: 2009 Dec |
Date Detail:
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Created Date: 2009-12-09 Completed Date: 2010-01-06 Revised Date: 2011-05-12 |
Medline Journal Info:
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Nlm Unique ID: 1273201 Medline TA: Eur J Immunol Country: Germany |
Other Details:
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Languages: eng Pagination: 3395-403 Citation Subset: IM |
Affiliation:
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Department of Immunology, University of Washington, Seattle, WA 98195, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals B-Lymphocytes / cytology, metabolism* Blotting, Western Calcium / metabolism Cell Cycle Cell Proliferation* Flow Cytometry G1 Phase Immunoglobulin G / metabolism* Membrane Glycoproteins / genetics, metabolism Mice Mice, Inbred C57BL Mice, Knockout NADPH Oxidase / genetics, metabolism Reactive Oxygen Species / metabolism Receptors, Antigen, B-Cell / physiology* Superoxides / metabolism* T-Lymphocytes / cytology, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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DE 16381/DE/NIDCR NIH HHS; GM37905/GM/NIGMS NIH HHS; R01 DE016381-05/DE/NIDCR NIH HHS; R01 GM037905-20/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin G; 0/Membrane Glycoproteins; 0/Reactive Oxygen Species; 0/Receptors, Antigen, B-Cell; 11062-77-4/Superoxides; 7440-70-2/Calcium; EC 1.6.3.1/Cybb protein, mouse; EC 1.6.3.1/NADPH Oxidase |
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