| BCR-ABL1 transcript levels increase in peripheral blood but not in granulocytes after physical exercise in patients with chronic myeloid leukemia. | |
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MedLine Citation:
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PMID: 20863169 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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In chronic myeloid leukemia (CML) treatment response is determined by measurements of BCR-AB1L transcripts in peripheral blood by quantitative real-time PCR (qRT-PCR) and a 2-5 fold increase is considered a warning sign. The BCR-ABL1 gene is mainly expressed in myeloid cells whereas quantification of BCR-ABL1 is performed on the nucleated cell fraction of peripheral blood. Hence, leukocyte composition of the nucleated cell fraction may affect the result of BCR-ABL1 quantification. The aim of this study was to investigate if changes in leukocyte composition of peripheral blood had any effect on BCR-ABL1 transcript levels in CML patients. Six CML patients in complete cytogenetic remission (CCgR) performed a maximal physical exercise test. Blood samples were collected before exercise, at maximal exhaustion and after exercise. A biphasic increase in leukocyte count was observed and the relative proportion of granulocytes in peripheral blood changed significantly after exercise compared with baseline (p < 0.001). The BCR-ABL1 transcript level increased significantly following exercise, in nucleated cell fraction of peripheral blood (p < 0.05) but not in isolated granulocytes. In the nucleated cell fraction, the mean BCR-ABL1 transcript level was 3.3-fold (range 0.7-6.8) higher 180 min after exercise compared with baseline (p < 0.01). In conclusion, physical exercise induced significant increases in BCR-ABL1 transcript levels concomitant with changes in leukocyte content of peripheral blood. We therefore suggest that variations in leukocyte composition of peripheral blood, causing pre-analytic variations that affect BCR-ABL1 quantification, have to be accounted for. Consequently, small variations in BCR-ABL1 transcript levels should be interpreted cautiously in CML patients in CCgR. |
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Authors:
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Sofia Jönsson; Bob Olsson; Stefan Jacobsson; Lars Palmqvist; Anne Ricksten; Kerstin Ekeland-Sjöberg; Hans Wadenvik |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-23 |
Journal Detail:
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Title: Scandinavian journal of clinical and laboratory investigation Volume: 71 ISSN: 1502-7686 ISO Abbreviation: Scand. J. Clin. Lab. Invest. Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-10 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0404375 Medline TA: Scand J Clin Lab Invest Country: England |
Other Details:
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Languages: eng Pagination: 7-11 Citation Subset: IM |
Affiliation:
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Section of Hematology, Department of Internal Medicine, Sahlgrenska University Hospital, University of Gothenburg, Sweden. sofi a.jonsson@vgregion.se |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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