Document Detail


The BB0646 protein demonstrates lipase and haemolytic activity associated with Borrelia burgdorferi, the aetiological agent of Lyme disease.
MedLine Citation:
PMID:  22151008     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The etiological agent of Lyme disease, Borrelia burgdorferi, is transmitted by ticks of the Ixodes genus and, if untreated, can cause significant morbidity in affected individuals. Recent reports have shown that polyunsaturated fatty acids in the B. burgdorferi cell envelope are potential targets for oxidative damage, which can be lethal. How B. burgdorferi responds to this assault is not known. Herein we report evidence that bb0646 codes for a lipase that is located within the bosR operon and that has specificity for both saturated and polyunsaturated fatty acids. Specifically, strains harbouring mutated copies of the lipase, either in the form of an insertionally inactivated construct or site-directed mutations within the active site, demonstrated attenuated lipolytic and haemolytic phenotypes when compared with the isogenic parent and trans-complements. In vivo analysis showed that while the bb0646 mutant remains infectious, the spirochaetal load is significantly lower than both the isogenic parent and the complemented mutant strains. Taken together, these data demonstrate that BB0646 is a broad substrate specific lipase that contributes to lipolytic and haemolytic activity in vitro and is required for optimal B. burgdorferi infection.
Authors:
Dana K Shaw; Jenny A Hyde; Jon T Skare
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2011-12-11
Journal Detail:
Title:  Molecular microbiology     Volume:  83     ISSN:  1365-2958     ISO Abbreviation:  Mol. Microbiol.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-01-11     Completed Date:  2012-05-01     Revised Date:  2014-09-10    
Medline Journal Info:
Nlm Unique ID:  8712028     Medline TA:  Mol Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  319-34     Citation Subset:  IM    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
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MeSH Terms
Descriptor/Qualifier:
Animal Structures / microbiology
Animals
Bacterial Load
Borrelia burgdorferi / enzymology*,  genetics
Disease Models, Animal
Fatty Acids / metabolism
Gene Knockout Techniques
Genetic Complementation Test
Hemolysin Proteins / genetics,  metabolism*
Lipase / genetics,  metabolism*
Lyme Disease / microbiology,  pathology
Mice
Mutagenesis, Insertional
Mutagenesis, Site-Directed
Mutation, Missense
Operon
Grant Support
ID/Acronym/Agency:
R01 AI042345/AI/NIAID NIH HHS; R01 AI042345-12/AI/NIAID NIH HHS; R01-AI042345/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Hemolysin Proteins; EC 3.1.1.3/Lipase
Comments/Corrections

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