Document Detail


BAC contig from a 3-cM region of mouse chromosome 11 surrounding Brca1.
MedLine Citation:
PMID:  11013085     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Even with the completion of a draft version of the human genome sequence only a fraction of the genes identified from this sequence have known functions. Chromosomal engineering in mouse cells, in concert with gene replacement assays to prove the functional significance of a given genomic region or gene, represents a rapid and productive means for understanding the role of a given set of genes. Both techniques rely heavily on detailed maps of chromosomal regions, initially to understand the scope of the regions being modified and finally to provide the cloned resources necessary to allow both finished sequencing and large insert complementation. This report describes the creation of a BAC clone contig on mouse chromosome 11 in a region showing conservation of synteny with sequences on human chromosome 17. We have created a detailed map of an approximately 3-cM region containing at least 33 genes through the use of multiple BAC mapping strategies, including chromosome walking and multiplex oligonucleotide hybridization and gap filling. The region described is one of the targets of a large effort to create a series of mice with regional deletions on mouse chromosome 11 (33-80 cM) that can subsequently be subjected to further mutagenesis.
Authors:
M Pershouse; J Li; C Yang; H Su; E Brundage; W Di; P J Biggs; A Bradley; A C Chinault
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Genomics     Volume:  69     ISSN:  0888-7543     ISO Abbreviation:  Genomics     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-10-27     Completed Date:  2001-01-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8800135     Medline TA:  Genomics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  139-42     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Academic Press.
Affiliation:
Department of Molecular and Human Genetics, Howard Hughes Medical Institute, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
BRCA1 Protein / genetics*
Chromosomes / genetics*
Chromosomes, Bacterial / genetics
Contig Mapping*
Expressed Sequence Tags
Mice
Mice, Inbred C57BL
Microsatellite Repeats
Grant Support
ID/Acronym/Agency:
CA75719/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/BRCA1 Protein

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